Abstract
The role of intracellular glutamine concentration in the regulation of14C-glutamine uptake was studied in a diazotrophic cyanobacteriumAnabaena 7120. The uptake pattern was found to be biphasic, consisting of a rapid first phase lasting up to 60 s followed by a slower second phase. Azaserine, which could not inhibit in vitro and in vivo glutamine synthetase (GS) activity effectively, inhibited the14C-glutamine uptake. Glutamine uptake was also not significantly affected when glutamate, methylglutamate, aspartate, arginine, lysine, hydroxylysine, ornithine, and GS inhibitor,L-methionine-DL-sulfoximine (MSX) were simultaneously available during uptake assay, suggesting that glutamine uptake takes place via a general amino acid permease which does not, however, transport basic and acidic amino acids. The azaserine-treated cells had increased and decreased levels of glutamine and glutamate, respectively, suggesting that the increased intracellular glutamine level is responsible for the inhibition of14C-glutamine uptake and provides evidence here for the role of an intracellular glutamine pool in the regulation of14C-glutamine uptake inAnabaena 7120.
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Literature Cited
Chapman JS, Meeks JC (1983) Glutamine and glutamate transport byAnabaena variabilis. J Bacteriol 156:122–129
Ferguson AR, Sims AP (1974) The regulation of glutamine metabolism inCandida utilis: the role of glutamine in control of glutamine synthetase. J Gen Microbiol 80:159–171
Flores E, Muro-Pastor I (1988) Uptake of glutamine and glutamate by the dinitrogen-fixing cyanobacteriumAnabaena sp. PCC 7120. FEMS Microbiol Lett 56:127–130
Flores E, Guerrero MG, Losada M (1980) Short-term ammonium inhibition of nitrate utilization ofAnacystis nidulans and other cyanobacteria. Arch Microbiol 128:137–144
Herrero A, Guerrero MG (1986) Regulation of nitrite reductase in the cyanobacteriumAnacystis nidulans. J Gen Microbiol 132:2463–2468
Herrero A, Flores E, Guerrero MG (1985) Regulation of nitrate reductase cellular levels in the cyanobacteriumAnabaena variabilis andSynechocystis sp. FEMS Microbiol Lett 26:21–25
Lea PJ, Norris RD (1976) the use of amino acid analogues in studies of plant metabolism. Phytochemistry 15:585–595
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin-phenol reagent. J Biol Chem 193:265–275
Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322
Merida A, Candau P, Florencio FJ (1991) Regulation of glutamine synthetase acttivity in the unicellular cyanobacteriumSynechocystis sp. PCC 6803 by the nitrogen source: effect of ammonium. J Bacteriol 173:4095–4100
Miflin BJ, Lea PJ (1976) The pathway of nitrogen assimilation in plants. Phytochemistry 15:873–885
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure culture of cyanobacteria. J Gen Microbiol 111:1–61
Rowell P, Enticott S, Stewart WDP (1977) Glutamine synthtase and nitrogenase activity in the blue-green algaAnabaena cylindrica. New Phytol 79:41–54
Sampaio MJAM, Rowell P, Stewart WDP (1979) Purification and some properties of glutamine synthetase from the nitrogenfixing cyanobacteriaAnabaena cylindrica andNostoc sp. J Gen Microbiol 111:181–191
Singh S (1988) Regulation of urea-uptake in the cyanobacteriumAnabaena doliolum. FEMS Microbiol Lett 56:281–284
Signh S (1990) Regulation of urease activity in the cyanobacteriumAnabaena doliolum. FEMS Microbiol Lett 67:79–84
Singh S (1991) Urea metabolism in the cyanobacteriumAnabaena cycadeae: regulation of urea uptake and urease by ammonia. FEMS Microbiol Lett 84:177–182
Singh S (1991) Involvement of ammonium assimilation in the ammonium inhibition of nitrogenase activity in cyanobacterium,Nostoc Anth. Indian J Exp Biol 29:496–497
Sing S (1992a) Regulation of urease cellular levels in the cyanobacteriaAnacystis nidulans andNostoc muscorum. Biochem Physiol Pflanz 188:33–38
Singh S (1992b) Nitrite metabolism in the cyanobacteriumAnabaena cycadeae: regulation of nitrite uptake and nitrite reductase by ammonia. FEMS Microbiol Lett 98:249–254
Singh S (1992c) Regulation of nitrite reductase cellular levels in the cyanobiontNostoc ANTH. Biochem Physiol Pflanz 188:241–246
Singh S (1992d) Regulation of nitrite uptake in the cyanobiontNostoc ANTH. Indian J Exp Biol 30:288–291
Singh S (1992e) Regulation of glutamine uptake in the cyanobiontNostoc ANTH. FEMS Microbiol Lett 98:245–248
Singh S (1993) Role of glutamine synthetase, glutamine and NH4 + in the regulation of glutamine uptake in the cyanobacteriumAnabaena cycadeae. J Gen Appl Microbiol 39:57–64
Singh S, Ahmad S (1989) Regulation of urea uptake by ammonia in the cyanobacteriumAnabaena doliolum. FEMS Microbiol Lett 61:199–202
Stewart WDP, Rowell P (1975) Effect ofL-methionine-DL-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production inAnabaena cylindrica. Biochem Biophys Res Commun 65:846–856
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Singh, S., Bisen, P.S. Evidence for the role of intracellular glutamine level in the regulation of glutamine uptake in the cyanobacteriumAnabaena 7120. Current Microbiology 29, 319–322 (1994). https://doi.org/10.1007/BF01570223
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DOI: https://doi.org/10.1007/BF01570223