Abstract
A new bacteriophage, designated F4, was isolated from the ruminal fluid of a calf. The host range of F4 phage was limited to five strains ofStreptococcus bovis out of ten tested on which clear plaques 0.6–1.2 mm in diameter were found. Bacteriophage F4 had an elongated head 75 nm long and 33 nm wide with a noncontractile flexible tail 100 nm in length on average. This phage is defective in the generation of plaques at low multiplicities of infection. Its genome consists of double-stranded linear DNA of 60.38 kb lacking cohesive ends. The F4 DNA was analyzed with 13 restriction enzymes. The restriction enzymes that did not cleave it wereBamHI,EcoRI,PvuI, andSmaI. The circular restriction map was constructed with four restriction endonucleases (XbaI,EcoI,SalI, andBglI).
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Literature Cited
Ackermann HW (1992) Frequency of morphological phage descriptions. Arch Virol 124:201–209
Ackermann HW, Cartier C, Slopek S, Vieu JF (1988) Morphology ofPseudomonas aeruginosa typing phages of the Lindberg set. Ann Microbiol (Paris) 139:389–404
Adams MH (1959) Bacteriophages. New York: Intersci Publishers
Bradley DE (1967) Ultrastructure of bacteriophages and bacteriocins. Bacteriol Rev 31:230–314
Iverson WG, Millis NF (1976) Characterization ofStreptococcus bovis bacteriophages. Can J Microbiol 22:847–852
Klieve AV, Bauchop T (1988) Morphological diversity of ruminal bacteriophages from sheep and cattle. Appl Environ Microbiol 54:1637–1641
Klieve AV, Gregg K, Bauchop T (1991) Isolation and characterization of lytic phages fromBacteroides ruminicola ss brevis. Curr Microbiol 23:183–187
Lockington RA, Attwood GT, Brooker JD (1988) Isolation and characterization of a temperate bacteriophage from the ruminal anaerobeSelenomonas ruminantium. Appl Environ Microbiol 54:1575–1580
Maniatis T, Fritsch EF, Sambrook JJ (1982) Molecular cloning laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Mrázek J, Španová A (1992) ANAGEL: a personal computer program for evaluating DNA fragment lengths from distances migrated in an agarose gel. CABIOS Appl Note 8:524
Noland-Jackson E, Miller HI, Adams ML (1978)EcoR I restriction endonuclease cleavage site map of bacteriophage P22 DNA. J Mol Biol 118:347–363
Paynter MJB, Ewert DL, Chalupa W (1969) Some morphological types of bacteriophages in bovine rumen contents. Appl Environ Microbiol 18:942–943
Španová A (1992) Comparison of permuted region lengths in the genomes of relatedSalmonella typhimurium phages P22 and L. Folia Microbiol 37:188–192
Štyriak I, Kmet' V, Španová A (1989) Isolation and characterization of two rumenStreptococcus bovis bacteriophages. Microbiologica 12:317–322
Štyriak I, Gálfi P, Kmet' V (1991) Preliminary observations of interaction between bacteriophages andStreptococcus bovis bacteria on ruminal epithelium primoculture. Vet Microbiol 29:281–287
Tamada H, Harasawa R, Shinjo T (1985) Isolation of a bacteriophage inFusobacterium necrophorum. Jpn J Vet Sci 47:483–486
Yamamoto KR, Alberts BM, Benzinger R, Lawhorne L, Treiber G (1970) Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to largescale virus purification. Virology 40:734–744
Zimmermann SB, Trach SO (1990) Quantitation of specific fragments in DNA restriction digests: application to the cohesive fragments in lambda DNA digests. Anal Biochem 188:267–270
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Štyriak, I., Španová, A., Montagová, H. et al. Isolation and characterization of a new ruminal bacteriophage lytic toStreptococcus bovis . Current Microbiology 28, 355–358 (1994). https://doi.org/10.1007/BF01570201
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DOI: https://doi.org/10.1007/BF01570201