Abstract
S. marcescens 8100 andP. aeruginosa 15442 were used to study bacterial adhesion to hydrogel contact lenses which had not been worn. Bacterial removal from unworn lens materials was assessed with a calibrated vortex device modified with a digital rpm readout and fitted with a test tube attachment (MVD). The MVD, which relies on a whirlpool-like force to remove the bacteria, showed that bacteria adhered to the same degree to etafilcon A, vifilcon A and polymacon lenses under standardized conditions. Tracking the isoenzyme patterns of these bacterial species over time showed instability ofS. marcescens upon repeated passage. This instability was not evident withP. aeruginosa. Bacterial adhesion ofP. aeruginosa 15442, to human worn and unworn etafilcon A materials was determined with a Modified Robbins Device. The MRD was closed off at both ends stopping medium and bacterial movement after 1 h of fluid flow over the lens surface. The results show that immediately following this 1-h period more bacteria adhere to unworn contact lenses than to worn lenses. However, bacterial counts were equivalent on worn and unworn lenses following 5 h of static incubation.
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References
Aswad MI, T John, M Barza, K Kenyon and J Baum. 1990. Bacterial adherence to extended wear soft contact lenses. Ophthalmology 97: 296–302.
Beltran P, JM Musser, R Helmuth, JJ Farmer III, WM Frerichs, IK Wachsmuth, K Ferris, AC McWhorter, JG Wells, A Cravioto and RK Selander. 1988. Toward a population genetic analysis ofSalmonella: genetic diversity and relationships among strains of serotypesS. choleraesuis, S. derby, S. dublin, S. enteritidis, S. heidelberg, S. infantis, S. newport, andS. typhimurium. PNAS (USA) 85: 7753–7757.
Boles SFB, MF Refojo and F-L Leong. 1992. Attachment ofPseudomonas to human-worn, disposable etafilcon A contact lenses. Cornea 11: 47–52.
Butrus SI, SA Klotz and RP Misra. 1987. The adherence ofPseudomonas aeruginosa to soft contact lenses. Ophthalmol 94: 1310–1314.
Dart JK and PR Badenoch. 1986. Bacterial adherence to contact lenses. CLAO 12: 220–224.
Dasgupta MK, K Lam, RA Ulan, KB Bettcher, V Burns, K Tyrrell, JB Dossetor, and JW Costerton. 1988. An extracorporeal model of biofilmadherent bacterial microcolony colonization for the study of peritonitis in continuous ambulatory peritoneal dialysis. Am J Nephrol 645: 1–6.
Dracopoli N and J Fogh. 1983. Polymorphic enzyme analysis of cultured human tumor cell lines. JNCI 70: 469–476.
Duran JA, MF Refojo, IK Gipson and KR Kenyon. 1987.Pseudomonas attachment to new hydrogel contact lenses. Arch Ophthalmol 105: 106–109.
Fleiszig SMJ, N Efron and GB Pier. 1992. Extended contact lens wear enhancesPseudomonas aeruginosa adherence to human corneal epithelium. Invest Ophthalmol Vis Sci 33: 2908–2916.
Fowler SA, JV Greiner and MR Allansmith. 1979. Attachment of bacteria to soft contact lenses. Arch Ophthalmol 97: 659–660.
Grimont PAD and F Grimont. 1984. Genus VIII. Serratia. In: Bergey's Manual of Systemic Bacteriology (Kreig NR, ed), pp 477–484, Williams and Wilkins, Baltimore.
Halton DM, WD Peterson Jr and B Hukku. 1983. Cell culture quality control by rapid isoenzymatic characterization. In Vitro 19: 16–24.
Hart DE, W Reindel, HM Proskin and MF Mowrey-Mckee. 1993. Microbial contamination of hydrophilic contact lenses: quantitation and identification of microorganisms associated with contact lenses while on the eye. Optom Vis Sci 70: 185–191.
Hart DE and KL Shih. 1987. Surface interactions on hydrogel extended wear contact lenses: microflora and microfauna. Am J Optom Phys Optics 64: 739–748.
Khoury AE, K Lam, B Ellis and JW Costerton. 1992. Prevention and control of bacterial infections associated with medical devices. ASAIO Journal 38: M174-M178.
Larwin-Brussel CA, MF Refojo, FL Leong, L Hanninen and KR Kenyon. 1993. Effect ofPseudomonas aeruginosa concentration in experimental contact lens-related microbial keratitis. Cornea 12: 10–18.
McCoy WF and JW Costerton. 1982. Fouling biofilm development in tubular flow systems. In: Developments in Industrial Microbiology, pp 551–558. Elsevier, Amsterdam.
McCoy WF, JD Bryers, J Robbins and JW Costerton. 1981. Observations of fouling biofilm formation. Can J Microbiol 27: 910–917.
Miller MJ and DG Ahearn. 1987. Adherence ofPseudomonas aeruginosa to hydrophilic contact lenses and other substrata. J Clin Microbiol 25: 1392–1397.
Miller MJ, LA Wilson and DG Ahearn. 1988. Effects of protein, mucin, and human tears on adherence ofPseudomonas aeruginosa to hydrophilic contact lenses. J Clin Microbiol 26: 513–517.
O'Brien SJ, JE Shannon and MH Gail. 1981. A molecular approach to the identification and individualization of human and animal cells in culture: isoenzyme and allozyme genetic signatures. In Vitro 16: 119–131.
Ottenbreit MJ, DM Halton and WD Peterson Jr, 1984. Rapid isoenzyme analysis of cell cultures by agarose electrophoresis II. Intraspecies identification of human cell lines. J Tis Cult Meth 8: 125–129.
Palleroni NJ. 1984. Genus I.Pseudomonas. In: Bergey's Manual of Systemic Bacteriology (Krieg NR, ed), pp 141–199, Williams and Wilkens, Baltimore.
Schultz CL. 1989. Isoenzyme analysis of typhoid-Shigella andEscherichia-Shigella hybrid vaccines and their parental strains. J Clin Microbiol 27: 12–17.
Stern GA and ZS Zam 1986. The pathogenesis of contact lens-associatedPseudomonas aeruginosa corneal ulceration. Cornea 5: 41–45.
United States Pharmacopeia XXII. 1990. The National Formulary. Rockville, MD. United States Pharmacopeial Convention, 1483–1488.
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Schultz, C.L., Pezzutti, M.R., Silor, D. et al. Bacterial adhesion measurements on soft contact lenses using a Modified Vortex Device and a Modified Robbins Device. Journal of Industrial Microbiology 15, 243–247 (1995). https://doi.org/10.1007/BF01569831
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DOI: https://doi.org/10.1007/BF01569831