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Influences on the antimicrobial activity of surface-adsorbed nisin

  • Published:
Journal of Industrial Microbiology

Abstract

The efficacy of the antimicrobial peptide nisin was examined after adsorption to silica surfaces. Three protocols were used to evaluate nisin's activity against adhered cells ofListeria monocytogenes: bioassay usingPediococcus pentosaceous FBB 61-2 as the sensitive indicator strain; visualization and enumeration of cells by microscopic image analysis; and viability of adhered cells as determined by lodonitrotetrazolium violet uptake and crystallization. The activity of adsorbed nisin was highly dependent upon conditions of adsorption. The highest antimicrobial activity of adsorbed nisin occurred with high concentrations of nisin (1.0 mg ml−1) and brief contact times (1 h) on surfaces of low hydrophobicity. Sequential adsorption of a second protein (β-lactoglobulin or bovine serum albumin) onto surfaces consistently resulted in decreased nisin activity. These data provide direction for the development of applications to limit microbial attachment on food contact surfaces through the use of adsorbed antimicrobial peptides.

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Authors and Affiliations

  1. Department of Food Science and Technology, Oregon State University, Wiegand Hall 100, 97331, Corvallis, Oregon, USA

    CK Bower, J McGuire & MA Daeschel

  2. Department of Bioresource Engineering, Oregon State University, Wiegand Hall 100, 97331, Corvallis, Oregon, USA

    J McGuire

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Bower, C., McGuire, J. & Daeschel, M. Influences on the antimicrobial activity of surface-adsorbed nisin. Journal of Industrial Microbiology 15, 227–233 (1995). https://doi.org/10.1007/BF01569829

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  • DOI: https://doi.org/10.1007/BF01569829

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