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Isolation and characterization of coaggregation-defective (Cog−) mutants ofStreptococcus gordonii DL1 (Challis)

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Journal of Industrial Microbiology

Abstract

Streptococcus gordonii DL1 (Challis) bears coaggregation-mediating surface adhesins which recognize galactoside-containing surface polysaccharides onStreptococcus oralis 34,Streptococcus oralis C104, andStreptococcus SM PK509. Fifty-nine spontaneously-occurring coaggregation-defective (Cog−) mutants ofS. gordonii DL1 unable to coaggregate with partner streptococci were isolated. Six representative Cog− mutants were characterized by their coaggregation properties with fourActinomyces naeslundii strains (T14V, PK947, PK606, PK984),Veillonella atypica PK1910, andPropionibacterium acnes PK93. The six representative Cog− mutants showed altered coaggregation with their streptococcal partners,A. naeslundii PK947, andP. acnes PK93. Based on the coaggregation phenotypes of these mutants, a model for the lactose-inhibitable coaggregation betweenS. gordonii DL1 and its partner bacteria is proposed. The potential use of these mutants in studies of oral biofilms is discussed.

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Authors and Affiliations

  1. Laboratory of Microbial Ecology, National Institute of Dental Research, National Institutes of Health, Building 30, Room 310, 30 Convent Dr MSC 4350, 20892, Bethesda, MD, USA

    D L Clemans & P E Kolenbrander PhD

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  1. D L Clemans
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  2. P E Kolenbrander PhD
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Clemans, D.L., Kolenbrander, P.E. Isolation and characterization of coaggregation-defective (Cog−) mutants ofStreptococcus gordonii DL1 (Challis). Journal of Industrial Microbiology 15, 193–197 (1995). https://doi.org/10.1007/BF01569825

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  • DOI: https://doi.org/10.1007/BF01569825

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