Journal of Industrial Microbiology

, Volume 15, Issue 3, pp 169–175 | Cite as

Dental plaque as a biofilm

  • P D Marsh
  • D J Bradshaw
Article

Abstract

Dental plaque is the diverse microbial community found on the tooth surface embedded in a matrix of polymers of bacterial and salivary origin. Once a tooth surface is cleaned, a conditioning film of proteins and glycoproteins is adsorbed rapidly to the tooth surface. Plaque formation involves the interaction between early bacterial colonisers and this film (the acquired enamel pellicle). To facilitate colonisation of the tooth surface, some receptors on salivary molecules are only exposed to bacteria once the molecule is adsorbed to a surface. Subsequently, secondary colonisers adhere to the already attached early colonisers (co-aggregation) through specific molecular interactions. These can involve protein-protein or carbohydrate-protein (lectin) interactions, and this process contributes to determining the pattern of bacterial succession. As the biofilm develops, gradients in biologically significant factors develop, and these permit the co-existence of species that would be incompatible with each other in a homogeneous environment. Dental plaque develops naturally, but it is also associated with two of the most prevalent diseases affecting industrialised societies (caries and periodontal diseases). Future strategies to control dental plaque will be targeted to interfering with the formation, structure and pattern of development of this biofilm.

Keywords

dental plaque biofilm adhesion co-aggregation 

References

  1. 1.
    Al-Hashimi I and MJ Levine. 1989. Characterization ofin vivo salivary-derived enamel pellicle. Archs Oral Biol 34: 289–295.Google Scholar
  2. 2.
    Berry CW and CA Henry. 1977. The effect of adsorption on the acid production of caries and non-caries-producing streptococci. J Dent Res 56: 1193–1200.PubMedGoogle Scholar
  3. 3.
    Bowden GHW. 1990. Microbiology of root surface caries in humans. J Dent Res 69: 1205–1210.PubMedGoogle Scholar
  4. 4.
    Bowden GHW. 1991. Which bacteria are cariogenic in humans? In: Dental Caries: Markers of High and Low Risk Groups and Individuals (Johnson NW, ed), pp 266–286, Cambridge University Press, Cambridge.Google Scholar
  5. 5.
    Bowden GHW, JM Hardie and GL Slack. 1975. Microbial variations in approximal dental plaque. Caries Res 9: 253–277.PubMedGoogle Scholar
  6. 6.
    Bradshaw DJ, KA Homer, PD Marsh and D Beighton. 1994. Metabolic co-operation in oral microbial communities during growth on mucin. Microbiol 140: 3407–3412.Google Scholar
  7. 7.
    Busscher HJ, MM Cowan and HC van der Mei. 1992. On the relative importance of specific and non-specific approaches to oral microbial adhesion. FEMS Microbiol Rev 88: 199–210.Google Scholar
  8. 8.
    Cisar JO. 1986. Fimbrial lectins of the oralActinomyces. In: Microbial Lectins and Aggultinins: Properties and Biological Activity (Mirelman D, ed), pp 183–196, John Wiley, New York.Google Scholar
  9. 9.
    Cisar JO, AK Sandberg and SE Mergenhagen. 1984. The function and distribution of different fimbriae on the strains ofActinomyces viscousus andActinomyces naeslundii. J Dent Res 63: 393–396.PubMedGoogle Scholar
  10. 10.
    Dibdin GH, CM Wilson and RP Shellis. 1983. Effect of packing density and polysaccharide to protein ratio of plaque samples culturedin vitro upon their permeability. Caries Res 17: 52–58.PubMedGoogle Scholar
  11. 11.
    Dibdin GH and RP Shellis. 1988. Physical and biochemical studies ofStreptococcus mutans sediments suggest new factors linking the cariogenicity of plaque with its extracellular polysaccharide content. J Dent Res 67: 890–895.PubMedGoogle Scholar
  12. 12.
    Douglas CWI, AA Pease and RA Whiley. 1990. Amylase-binding as a discriminator among oral streptococci. FEMS Microbiol Lett 66: 193–198.Google Scholar
  13. 13.
    Genco RJ and J Slots. 1984. Host responses in periodontal diseases. J Dent Res 63: 441–451.PubMedGoogle Scholar
  14. 14.
    George KS and WA Falkler Jr. 1992. Coaggregation studies of theEubacterium species. Oral Microbiol Immunol 7: 285–290.PubMedGoogle Scholar
  15. 15.
    Gibbons RJ, 1989. Bacterial adhesion to oral tissues: a model for infectious diseases. J Dent Res 68: 750–760.PubMedGoogle Scholar
  16. 16.
    Gibbons RJ, DI Hay, JO Cisar and WB Clark. 1988. Adsorbed salivary proline-rich protein-1 and statherin: receptors for type 1 fimbriae ofActinomyces viscousus T14V-J1 on apatitic surfaces. Infect Immun 56: 2990–2993.PubMedGoogle Scholar
  17. 17.
    Gibbons RJ, DI Hay, WC Childs III and G Davis. 1990. Role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces. Arch Oral Biol 35 (suppl): 107S-114S.PubMedGoogle Scholar
  18. 18.
    Gibbons RJ, DI Hay and DH Schlesinger. 1991. Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion ofStreptococcus gordonii to apatitic surfaces. Infect Immun 59: 2948–2954.PubMedGoogle Scholar
  19. 19.
    Guggenheim B and R Schmid. 1989. Chemical plaque control: whatin vitro and animal test systems are appropriate? J Dent Res 68: 1645–1654.Google Scholar
  20. 20.
    Handley PS, LM Hesketh and RA Moumena. 1991. Charged and hydrophobic groups are localised in the short and long tuft fibrils onStreptococcus sanguis strains Biofouling. 4: 105–111.Google Scholar
  21. 21.
    Hudson MC and R Curtiss III. 1990. Regulation of expression ofStreptococcus mutans genes important to virulence. Infect Immun 58: 464–470.PubMedGoogle Scholar
  22. 22.
    Jenkinson HF, SD Terry, R McNab and GW Tannock. 1993. Inactivation of the gene encoding surface protein SspA inStreptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oralActinomyces. Infect Immun 61: 3199–3208.PubMedGoogle Scholar
  23. 23.
    Kolenbrander PE, RN Andersen and LVM Moore. 1989. Coaggregation ofFusobacterium nucleatum, Selenomonas flueggei, Selenomonas infelix, Selenomonas noxia, andSelenomonas sputigena with strains from 11 genera of oral bacteria. Infect Immun 57: 3194–3203.PubMedGoogle Scholar
  24. 24.
    Kolenbrander PE and J London. 1992. Ecological significance of coag-gregation among oral bacteria. Adv Microb Ecol 12: 183–217.Google Scholar
  25. 25.
    Kolenbrander PE and J London. 1993. Adhere today, here tomorrow: oral bacterial adherence. J Bacteriol 175: 3247–4252.PubMedGoogle Scholar
  26. 26.
    Listgarten MA. 1976. Structure of the microbial flora associated with periodontal health and disease in man. A light and electron microscopic study. J Periodontol 47: 1–18.PubMedGoogle Scholar
  27. 27.
    Loesche WJ. 1986. Role ofStreptococcus mutans in human dental decay. Microbiol Rev 50: 353–380.PubMedGoogle Scholar
  28. 28.
    Marsh PD. 1989. Host defences and microbial homeostasis: role of microbial interactions. J Dent Res 68: 1567–1575.Google Scholar
  29. 29.
    Marsh PD. 1993. Microbiological aspects of the chemical control of plaque and gingivitis. J Dent Res 71: 1431–1438.Google Scholar
  30. 30.
    Marsh PD and DJ Bradshaw. 1993. Microbiological effects of new agents in dentrifrices for plaque control. Int Dent J 43: 399–406.PubMedGoogle Scholar
  31. 31.
    Marsh PD and MV Martin. 1992. Oral Microbiology, 3rd edn. Chapman and Hall, London.Google Scholar
  32. 32.
    Marsh PD, A Featherstone, AS McKee, C Hallsworth, C Robinson, JA Weatherell, HN Newman and AFV Pitter. 1989. A microbiological study of early caries of approximal surfaces in schoolchildren. J Dent Res 68: 1151–1154.PubMedGoogle Scholar
  33. 33.
    McBride BC and MT Gislow. 1977. Role of sialic acid in saliva induced aggregation ofStreptococcus sanguis. Infect Immun 18: 35–40.PubMedGoogle Scholar
  34. 34.
    McNee SG, DAM Geddes, DA Weetman, D Sweeney and JA Beeley. 1982. Effect of extracellular polysaccharides on diffusion of NaF and14C-sucrose in human dental plaque and in sediments of the bacteriumStreptococcus sanguis 804 (NCTC 10904). Arch Oral Biol 27:981–986.PubMedGoogle Scholar
  35. 35.
    Meikle MC, JK Heath and JJ Reynolds. 1986. Advances in understanding cell interactions in tissue resorption. Relevance to the pathogenesis of periodontal diseases and a new hypothesis. J Oral Path 15: 239–250.PubMedGoogle Scholar
  36. 36.
    Millward TA and M Wilson. 1989. The effect of chlorhexidine onStreptococcus sanguis biofilms. Microbios 58:155–164.PubMedGoogle Scholar
  37. 37.
    Moore WEC, LV Holdeman, EP Cato, RM Smibert, JA Burmeister, and RR Ranney. 1983. Bacteriology of moderate (chronic) periodontitis in mature adult humans. Infect Immun 42: 510–515.PubMedGoogle Scholar
  38. 38.
    Moore LVH, WEC Moore, EP Cato, RM Smibert, JA Burmeister, AM Best and RR Ranney. 1987. Bacteriology of human gingivitis. J Dent Res 66: 989–995.PubMedGoogle Scholar
  39. 39.
    Murray PA, MJ Levine, LA Tabak and MS Reddy. 1982. Specificity of salivary-bacterial interactions. II. Evidence for a lectin onStreptococcus sanguis with specificity for a NeuAc (α)2,3Gal(β)1, 3GalNAc sequence. Biochem Biophys Res Comm 106: 390–396.PubMedGoogle Scholar
  40. 40.
    Murray PA, MJ Levine, MS Reddy, LA Tabak and EJ Bergey. 1986. Preparation of a sialic acid-binding protein fromStreptococcus mitis KS32AR. Infect Immun 53: 359–365.PubMedGoogle Scholar
  41. 41.
    Nyvad B. 1993. Microbial colonization of human tooth surfaces. APMIS 101 (Suppl 32): 7–45.Google Scholar
  42. 42.
    Nyvad B and O Fejerskov. 1989. Structure of dental plaque and the plaque-enamel interface in human experimental caries. Caries Res 23: 151–158.PubMedGoogle Scholar
  43. 43.
    Nyvad B and M Kilian. 1987. Microbiology of the early colonization of human enamel and root surfacesin vivo. Scand J Dent Res 95: 369–380.PubMedGoogle Scholar
  44. 44.
    Nyvad B and M Kilian. 1990. Comparison of the initial streptococcal microflora on dental enamel in caries-active and in caries-inactive individuals. Caries Res 24: 267–272.PubMedGoogle Scholar
  45. 45.
    Reinholdt J and M Kilian. 1987. Interference of IgA protease with the effect of secretory IgA on adherence of oral streptococci to salivacoated hydroxyapatite. J Dent Res 66: 492–497.PubMedGoogle Scholar
  46. 46.
    Rutter PR and B Vincent. 1980. The adhesion of microorganisms to surfaces: physico-chemical aspects. In: Microbial Adhesion to Surfaces (Berkeley RCW, JM Lynch, J Melling, PR Rutter and B Vincent, eds), pp 79–93. Ellis Horwood, Chichester.Google Scholar
  47. 47.
    Savitt ED and SS Socransky. 1984. Distribution of certain subgingival microbial species in selected periodontal conditions. J Perio Res 19: 111–123.Google Scholar
  48. 48.
    Scannapieco FA, EJ Bergey, MS Reddy and MJ Levine. 1989. Characterization of salivary α-amylase binding toStreptococcus sanguis. Infect Immun 57: 2853–2863.PubMedGoogle Scholar
  49. 49.
    Scheie AAa. 1989. Modes of action of currently known chemical antiplaque agents other than chlorhexidine. J Dent Res 68: 1609–1616.Google Scholar
  50. 50.
    Scheie AAa, KH Eggen and R Rölla. 1987. Glucosyltransferase activity in humanin vivo formed enamel pellicle and in whole saliva. Scand J Dent Res 95: 212–215.PubMedGoogle Scholar
  51. 51.
    Schilling KM and WH Bowen. 1988. The activity of glucosyltransferase adsorbed onto saliva-coated hydroxyapatite. J Dent Res 67: 2–8.PubMedGoogle Scholar
  52. 52.
    Shibata S, K Nagata, R Nakamura, A Tsunemitsu and A Misaki. 1980. Interaction of parotid saliva basic glycoprotein withStreptococcus sanguis ATCC 10557. J Periodontol 51: 499–504.PubMedGoogle Scholar
  53. 53.
    Slots J. 1977. Microflora in the healthy gingival sulcus in man. Scand J Dent Res 85: 247–254.PubMedGoogle Scholar
  54. 54.
    Slots J and RJ Genco. 1984. Black-pigmentedBacteroides species,Capnocytophaga species, andActinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. J Dent Res 63: 412–421.PubMedGoogle Scholar
  55. 55.
    Socransky SS, AD Haffajee, JM Goodson and J Lindhe. 1984. New concepts of destructive periodontal disease. J Clin Periodontol 11: 21–31.PubMedGoogle Scholar
  56. 56.
    Strömberg N and T Borén. 1992.Actinomyces tissue specificity may depend on differences in receptor specificity for GalNAcβ-containing glycoconjugates. Infect Immun 60: 3268–3277.PubMedGoogle Scholar
  57. 57.
    Strömberg N, T Borén, A Carlén and J Olsson. 1992. Salivary receptors for GalNAcβ-sensitive adherence ofActinomyces spp: evidence for heterogeneous GalNAcβ and proline-rich receptor properties. Infect Immun 60: 3278–3286.PubMedGoogle Scholar
  58. 58.
    Theilade E, O Fejerskov, W Prachyabrued and M Kilian. 1974. Microbiologic study on developing plaque in human fissures. Scand J Dent Res 82: 420–427.PubMedGoogle Scholar
  59. 59.
    Theilade E, O Fejerskov, T Karring and J Theilade. 1982. Predominant cultivable microflora of human dental fissure plaque. Infect Immun 36: 977–982.PubMedGoogle Scholar
  60. 60.
    van Palenstein Helderman WH. 1975. Total viable count and differential count ofVibrio (Campylobacter) sputorum, Fusobacterium nucleatum, Selenomonas sputigena, Bacteroides ochraceus andVeillonella in the inflamed and non inflamed gingival crevice. J Periodont Res 10: 230–241.PubMedGoogle Scholar
  61. 61.
    Walker GJ and NA Jacques. 1987. Polysaccharides of oral streptococci. In: Sugar Transport and Metabolism in Gram-positive Bacteria (Reizer A and A Peterkofsky, eds), pp 39–68, Ellis Horwood, Chichester.Google Scholar
  62. 62.
    Zero DT, J van Houte and J Russo. 1986. The intraoral effect on enamel demineralization of extracellular matrix material synthesized from sucrose byStreptococcus mutans. J Dent Res 65: 918–923.PubMedGoogle Scholar

Copyright information

© Society for Industrial Microbiology 1995

Authors and Affiliations

  • P D Marsh
    • 1
  • D J Bradshaw
    • 1
  1. 1.Department of Microbial PathogenicityCAMRSalisburyUK

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