Abstract
Four colony variants (two stable and two unstable phenotypes) were observed inBacillus subtilis α-10, an α-amylase-hyperproducing strain. The stable variants lost the ability to produce α-amylase, while the unstable ones reverted to the typical morphology after restreaking. Unstable expanding sectors appeared in typical colonies, and their appearance was influenced by the culture origin and age.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alea F, AM Ibañez and A Martinez. 1989. Producción de α-amilasa en cepas deBacillus subtilis. Rev ICIDCA 23: 4–10.
Buchanan RE and NE Gibbons. 1984. Bergey's Manual of Determinative Bacteriology, 8th edn (Krieg NR, ed), Williams & Wilkins, Baltimore.
Cadmus MC, P Rogovin and KA Burton. 1976. Colonial variation inXanthomonas campestris NRRL-B 1459 and characterization of exopolysaccharide from a variant strain. Can J Microbiol 22: 942–948.
Carlisle GE and JO Falkinhan III. 1989. Enzyme activities and antibiotic susceptibility of colonial variants ofBacillus subtilis andBacillus liqueniformis. Appl Environ Microbiol 55: 3026–3028.
Maniatis T, EF Fritsch and J Sambrook. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.
Marsalková JA. 1982. Characteristic morphological features of high-production isolates of monensin producerStreptomyces cinnamonenses. Folia Microbiol 27: 323–327.
Martínez JP, ML Gil, M Casanova, JL López-Ribot, J García de Lomas and R Sentandreu. 1990. Wall mannoproteins in cells from colonial phenotypic variants ofCandida albicans. J Gen Microbiol 136: 2421: 2432.
Perlman D, RB Greenfield Jr and A O'Brien. 1954. Degeneration ofStreptomyces griseus mutant on repeated transfer. Appl Microbiol 2: 199–202.
Shapiro JA. 1988. What transposable elements do in bacteria. In: Eukariotic Transposable Elements as Mutagenic Agents (Lambert ME, JF McDonald and IB Weinstein, eds), pp 3–15, Cold Spring Harbor Laboratory. Cold Spring Harbor, New York.
Shapiro JA and NP Higgins. 1989. Differential activity of a transposable element inEscherichia coli colonies. J Bacteriol 171: 5975–5986.
Singer M and P Berg. 1991. Genes and Genomes. A Changing Perspective (Singer M and P Berg, eds), pp 733–737, University Science Books, Mill Valley, California.
Soll DR. 1989. High-frequency switching inCandida albicans. In: Mobile DNA (Berg DE and MM Howe, eds), pp 791–799, Amer Soc for Microbiology, Washington DC.
Swanson J and JM Koone. 1989. Mechanisms for variation of pili and outer membrane protein II inNeisseria gonorrhoeae. In: Mobile DNA Berg DE and MM Howe, eds), pp 743–761, Amer Soc for Microbiology, Washington DC.
Tichy P, J Pazlarová, M Hartman, Z Fencl, L Erbenová, O Benada and V Krumphanzl. 1981. Isolation of the pM110 plasmid from the α-amylase producing strain ofBacillus subtilis. Mol Gen Genet 181: 248–254.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rodríguez, H. Colony switching in an alpha-amylase-producing strain ofBacillus subtilis . Journal of Industrial Microbiology 15, 112–115 (1995). https://doi.org/10.1007/BF01569809
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01569809