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Microbial transformation ofN-heptyl physostigmine, a semisynthetic alkaloid inhibitor of cholinesterase

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Journal of Industrial Microbiology

Abstract

The microbiological transformation ofN-heptyl physostigmine (L-693, 487) (1), a semisynthetic physostigmine cholinesterase inhibitor, was investigated usingVerticillium lecanii MF 5713 (ATCC 74148),Acremonium sp MF 5723 (ATCC 74164) andActinoplanes sp MA 6559 (ATCC 53771). Nine microbial metabolites (2–10) of 1 were isolated and purified using reversed-phase HPLC. The structures of the metabolites were established using spectroscopic techniques including MS and NMR. Some of the microbial metabolites were identical to metabolites present in urine of a dog treated with 1.

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Authors and Affiliations

  1. Department 807, Department of Fermentation Microbiology, Merck Research Laboratories, Building R80Y-205, 07065, Rahway, NJ, USA

    L So, R White, B Arison, B Petuch, R Monaghan & T S Chen PhD

  2. Department of Drug Metabolism, 19486, West Point, PA, USA

    M S Schwartz & H Cheng

Authors
  1. L So
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  2. R White
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  3. B Arison
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  4. B Petuch
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  5. M S Schwartz
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  6. H Cheng
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  7. R Monaghan
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  8. T S Chen PhD
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So, L., White, R., Arison, B. et al. Microbial transformation ofN-heptyl physostigmine, a semisynthetic alkaloid inhibitor of cholinesterase. Journal of Industrial Microbiology 15, 108–111 (1995). https://doi.org/10.1007/BF01569808

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  • DOI: https://doi.org/10.1007/BF01569808

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