Skip to main content
Log in

Sequence of the gene encoding the 16S rRNA of the beer spoilage organismMegasphaera cerevisiae

  • Published:
Journal of Industrial Microbiology

Abstract

The 16S ribosomal RNA gene from the beer-spoilage organism,Megasphaera cerevisiae was polymerase chain reaction (PCR)-amplified and sequenced. Analysis confirmed the phylogenetic position ofM. cerevisiae as a sister taxon ofMegasphaera elsdenii, within the obligately anaerobic, Gram-negative cocci. The sequence obtained should facilitate the development of DNA probes for early detection of this spoilage organism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Chelack BJ and WM Ingledew. 1987. Anaerobic Gram-negative bacteria in brewing—a review. J Amer Soc Brew Chem 45: 123–127.

    Google Scholar 

  2. DiMichele L and MJ Lewis. 1993. Rapid, species specific detection of lactic acid bacteria from beer using the polymerase chain reaction. J Amer Soc Brew Chem 51: 63–66.

    Google Scholar 

  3. Engelmann U and N Weiss. 1985.Megasphaera cerevisiae sp nov: a new Gram-negative obligately anaerobic coccus isolated from spoiled beer. Syst Appl Microbiol 6: 287–290.

    Google Scholar 

  4. Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.

    Google Scholar 

  5. Felsenstein J. 1993. PHYLIP version 3.5c: phylogeny inference package. Distributed by author. University of Washington, Seattle.

    Google Scholar 

  6. Haikara A. 1991. The genusPectinatus andMegasphaera. In: The Prokaryotes, vol II, 2nd edn (Balows A, HG Trüper, M Dworkin, W Harder and K-H Schleifer, eds), pp 1993–2004, Springer-Verlag, New York.

    Google Scholar 

  7. Haikara A and K Lounatmaa. 1987. Characterization ofMegasphaera sp, a new anaerobic beer spoilage coccus. Proc Congr Eur Brew Conv 23: 473–480.

    Google Scholar 

  8. Higuchi RG and H Ochman. 1989. Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction. Nucl Acids Res 17: 5865.

    PubMed  Google Scholar 

  9. Jukes TH and CR Cantor. 1969. Evolution of protein molecules. In: Mammalian Protein Metabolism (Munro HN, ed), pp 21–132, Academic Press, New York.

    Google Scholar 

  10. Olsen GJ, N Larsen and CR Woese. 1991. The ribosomal RNA database project. Nucl Acids Res 19: 2017–2018.

    PubMed  Google Scholar 

  11. Saitou N and M Nei. 1987. The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4: 406–425.

    PubMed  Google Scholar 

  12. Schleifer KH, M Leuteritz, N Weiss, W Ludwig, G Kirchhof and H Seidel-Rufer. 1990. Taxonomic study of anaerobic, Gram-negative, rod-shaped bacteria from breweries: emended description ofPectinatus cerevisiiphilus and description ofPectinatus frisingensis sp nov,Selenomonas lactifex sp nov,Zymophilus raffinosivorans gen nov, sp nov, andZymophilus paucivorans gen nov, sp nov. Int J Syst Bacteriol 40: 19–27.

    PubMed  Google Scholar 

  13. Swofford DL. 1993. PAUP version 3.1.1: phylogenetic analysis using parsimony. Distributed by author, Smithsonian Institute. Washington, DC.

    Google Scholar 

  14. Tsuchiya Y, H Kaneda, Y Kano and S Koshino. 1992. Detection of beer spoilage organisms by polymerase chain reaction technology. J Amer Soc Brew Chem 50: 64–67.

    Google Scholar 

  15. Tsuchiya Y, Y Kano and S Koshino. 1993. Detection ofLactobacillus brevis in beer using polymerase chain reaction technology. J Amer Soc Brew Chem 51: 40–41.

    Google Scholar 

  16. Weiss N, H Seidel and W Back. 1979. Isolierung und systematische Zuordnung biershädlicher gramnegativer Bakterien. I: Gramnegative strikt anaerobe Kokken. Brauwissenschaft 32: 189–194.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Doyle, L.M., McInerney, J.O., Mooney, J. et al. Sequence of the gene encoding the 16S rRNA of the beer spoilage organismMegasphaera cerevisiae . Journal of Industrial Microbiology 15, 67–70 (1995). https://doi.org/10.1007/BF01569801

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01569801

Keywords

Navigation