Abstract
The 16S ribosomal RNA gene from the beer-spoilage organism,Megasphaera cerevisiae was polymerase chain reaction (PCR)-amplified and sequenced. Analysis confirmed the phylogenetic position ofM. cerevisiae as a sister taxon ofMegasphaera elsdenii, within the obligately anaerobic, Gram-negative cocci. The sequence obtained should facilitate the development of DNA probes for early detection of this spoilage organism.
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Chelack BJ and WM Ingledew. 1987. Anaerobic Gram-negative bacteria in brewing—a review. J Amer Soc Brew Chem 45: 123–127.
DiMichele L and MJ Lewis. 1993. Rapid, species specific detection of lactic acid bacteria from beer using the polymerase chain reaction. J Amer Soc Brew Chem 51: 63–66.
Engelmann U and N Weiss. 1985.Megasphaera cerevisiae sp nov: a new Gram-negative obligately anaerobic coccus isolated from spoiled beer. Syst Appl Microbiol 6: 287–290.
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.
Felsenstein J. 1993. PHYLIP version 3.5c: phylogeny inference package. Distributed by author. University of Washington, Seattle.
Haikara A. 1991. The genusPectinatus andMegasphaera. In: The Prokaryotes, vol II, 2nd edn (Balows A, HG Trüper, M Dworkin, W Harder and K-H Schleifer, eds), pp 1993–2004, Springer-Verlag, New York.
Haikara A and K Lounatmaa. 1987. Characterization ofMegasphaera sp, a new anaerobic beer spoilage coccus. Proc Congr Eur Brew Conv 23: 473–480.
Higuchi RG and H Ochman. 1989. Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction. Nucl Acids Res 17: 5865.
Jukes TH and CR Cantor. 1969. Evolution of protein molecules. In: Mammalian Protein Metabolism (Munro HN, ed), pp 21–132, Academic Press, New York.
Olsen GJ, N Larsen and CR Woese. 1991. The ribosomal RNA database project. Nucl Acids Res 19: 2017–2018.
Saitou N and M Nei. 1987. The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4: 406–425.
Schleifer KH, M Leuteritz, N Weiss, W Ludwig, G Kirchhof and H Seidel-Rufer. 1990. Taxonomic study of anaerobic, Gram-negative, rod-shaped bacteria from breweries: emended description ofPectinatus cerevisiiphilus and description ofPectinatus frisingensis sp nov,Selenomonas lactifex sp nov,Zymophilus raffinosivorans gen nov, sp nov, andZymophilus paucivorans gen nov, sp nov. Int J Syst Bacteriol 40: 19–27.
Swofford DL. 1993. PAUP version 3.1.1: phylogenetic analysis using parsimony. Distributed by author, Smithsonian Institute. Washington, DC.
Tsuchiya Y, H Kaneda, Y Kano and S Koshino. 1992. Detection of beer spoilage organisms by polymerase chain reaction technology. J Amer Soc Brew Chem 50: 64–67.
Tsuchiya Y, Y Kano and S Koshino. 1993. Detection ofLactobacillus brevis in beer using polymerase chain reaction technology. J Amer Soc Brew Chem 51: 40–41.
Weiss N, H Seidel and W Back. 1979. Isolierung und systematische Zuordnung biershädlicher gramnegativer Bakterien. I: Gramnegative strikt anaerobe Kokken. Brauwissenschaft 32: 189–194.
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Doyle, L.M., McInerney, J.O., Mooney, J. et al. Sequence of the gene encoding the 16S rRNA of the beer spoilage organismMegasphaera cerevisiae . Journal of Industrial Microbiology 15, 67–70 (1995). https://doi.org/10.1007/BF01569801
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DOI: https://doi.org/10.1007/BF01569801