Summary
A new insoluble chromogenic substrate for the determination of proteolytic activity was developed. This substrate was prepared by incorporating black drawing ink into casein and heating this complex at 200°C for 4 h. It is especially suitable for determining the activity of alkaline bacterial proteinases.
This is a preview of subscription content, log in via an institution to check access.
References
Fritz, H., U. Seemüller and H. Tschesche. 1984. Proteinases and their inhibitors. General review. In: Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.), pp. 74–98, Verlag Chemie, Weinheim.
Fukal, L. and J. Káš. 1984. Review of techniques for assaying proteolytic enzymes (in Czech). Chem. Listy 78: 1176–1196.
Technický Naučný Slovník (Technical Encyclopedia). 1985. Part VI, p. 568, SNTL, Praha.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Šafařík, I. An inexpensive insoluble chromogenic substrate for the determination of proteolytic activity. Journal of Industrial Microbiology 3, 259–261 (1988). https://doi.org/10.1007/BF01569584
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01569584