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Heat-induced injury inListeria monocytogenes

  • Original Papers
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Journal of Industrial Microbiology

Summary

Heating ofListeria monocytogenes (Scott A strain) in potassium phosphate buffer (0.1 M, pH 7.2) at 52°C for 1 h led to injury, with the heat-injured cells failing to produce colonies on agar medium containing 5% NaCl. The detection of injury was based on the use of differential media: plating on tryptose phosphate broth+2% agar and 1% sodium pyruvate (TPBA+P) and on tryptose phosphate broth+2% agar and 5% NaCl (TPBA+S). Only non-injuredListeria formed colonies on TPBA+S whereas both heat-injured and non-injured cells formed colonies on TPBA+P. The bacterial count on TPBA+P minus that on TPBA+S represents the extent of heat injury. A large number of selective agars were tested and compared to TPBA+P for their ability to support repair and colony formation of heat-injuredL. monocytogenes. Media containing 0.025% phenylethanol, 0.0012–0.0025% acriflavin, 0.1–0.2% potassium tellurite, 0.001% polymyxin B sulfate, 5% NaCl or a combination of these ingredients were detrimental to the recovery of heat-injuredL. monocytogenes. Media currently in use forL. monocytogenes are not satisfactory for the recovery of injured cells.

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Authors and Affiliations

  1. Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, 19118, Philadelphia, PA, USA

    J. L. Smith & D. L. Archer

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  1. J. L. Smith
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  2. D. L. Archer
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Smith, J.L., Archer, D.L. Heat-induced injury inListeria monocytogenes . Journal of Industrial Microbiology 3, 105–110 (1988). https://doi.org/10.1007/BF01569551

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  • DOI: https://doi.org/10.1007/BF01569551

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