Lactic acid production by an isolated ofLactobacillus plantarum was standardised on enzyme-hydrolysed tapioca (Manihot esculenta) flour, tapioca starch and soluble starch. Calculated yields of lactic acid (g from 100 g reducing sugars used) in nutrient media containing the abovementioned hydrolysates (10% reducing sugars) were 21.8%, 16.2% and 16.2%, respectively. Higher yields (29–34%) were obtained in media containing 5% reducing sugars. A conversion efficiency of 80–99% was achieved when the acid produced in the broth was neutralised periodically. One hundred milliliters of the medium (5% sugars) yielded 4.0–4.5 g of calcium lactate. These results indicate that unrefined starchy material can be successfully employed for the economic production of lactic acid. The same substrate can also be utilised for biomass production, as viable lactobacilli are being used for therapy in medicine.
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Aksu, Z. and T. Kutsal. 1986. Lactic acid production from molasses utilizingL. delbrückii. Biotechnol. Lett. 8: 157–160.
Anon. 1986. All India crop estimate 1984–85. Food Digest. 9: 113.
Block, R.J., E.L. Durrum and G. Zweig. 1955. Aliphatic acids. In: A Manual of Paper Chromatography and Paper Electrophoresis, pp. 157–182, Academic Press Inc., New York.
Miller, G.L. 1959. Use of dinitrosalicylic acid as reagent for the determination of reducing sugars. Anal. Chem. 31: 426–428.
Prescott, S.C. and C.G. Dunn. 1959. The production of lactic acid by fermentation. In: Industrial Microbiology, pp. 304–331, McGraw-Hill Book Co. Inc., New York.
Ratledge, C. 1977. Fermentation substrates. In: Annual Reports on Fermentation Processes (Perlman, D., ed.), Vol. 1, p. 49, Academic Press, New York.
Rogosa, M. 1974.Lactobacillus. In: Bergey's Manual of Determinative Bacteriology (Buchanan, R.E. and N.E. Gibbons, eds.), 8th Edn., pp. 576–593, The Williams and Wilkins Co., Baltimore.
Whittier, E.O. and B.H. Webb. 1950. Fermentation products from whey. In: Byproducts from Milk, pp. 31–49, Reinhold Publishing Co., New York.
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Shamala, T.R., Sreekantiah, K.R. Fermentation of starch hydrolysates byLactobacillus plantarum . Journal of Industrial Microbiology 3, 175–178 (1988). https://doi.org/10.1007/BF01569524
- Bacterial α-amylase
- Starch hydrolysate
- Lactobacillus plantarum
- Lactic acid fermentation