Summary
The potential for changes in allele frequencies in yeast populations by selection was examined. Cells from the wine yeastSaccharomyces cerevisiae (strain Montrachet) were grown over a large number of generations using two different culturing techniques, each with two variations: serial transfers on WLN agar plates with and without UV irradiation, and continuous culture in autoclaved and in filter-sterilized grape must. A low frequency of variant isozyme patterns was found in samples taken at the end of the experiment. Growth rates in must and on agar plates were also examined, and it was found that all samples were faster-growing than the original strain, to varying degrees. Applications for the selection system developed are discussed.
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References
Alikhanyan, S.I. and G.M. Nalbandyan. 1971. Selection of wine yeasts with the use of mutagens. I. Selection ofSaccharomyces cerevisiae strains used in the production of natural strong table wines. Sov. Genet. 7: 1200–1205.
Amerine, M.A. and R.E. Kunkee. 1968. Microbiology of winemaking. Annu. Rev. Microbiol. 22: 323–358.
Bauer, von H. and J. Kleinhenz. 1978. Technologische Kenngroessen von Trochenhefen. Wein Wiss. 3: 188–199.
Carrau, J.L., J.L. de Azevedo, P. Siedbery and D. Campbell. 1982. Methods for recovering fusion products among oenological strains ofSaccharomyces cerevisiae andSchizosaccharomyces pombe. Rev. Brasil Genet. 1: 221–226.
Clayton, J.W. and D.N. Tretiak. 1972. Amino-citrate buffers for pH control in starch gel electrophoresis. J. Fish. Res. Board Can. 29: 1169–1172.
Davenport, R.R. 1974. Microecology of yeasts and yeast-like organisms associated with an English vineyard. Vitis 13: 123–130.
Delfini, C. and G. Ciolfi. 1980. Setting up standard methods to assign the oenological peculiarities of selected yeasts. III. The determination of the capability of degrading malic acid by maloalcoholic fermentation. Vini Ital. 22: 301–308.
Eschenbruch, R., K.J. Cressel, B.M. Fisher and R.J. Thornton. 1982. Selective hybridization of pure culture wine yeasts. I. Elimination of undesirable winemaking properties. Eur. J. Appl. Microbiol. Biotechnol. 14: 155–158.
Eschenbruch, R. and J.M. Rassell. 1975. The development of non-foaming yeast strains for wine-making. Vitis 14(1): 43–47.
Harris, H. and D.A. Hopkinson. 1976. Handbook of enzyme electrophoresis in human genetics. Elsevier/North-Holland Publishing Co., Amsterdam.
Irwin, D., R.E. Subden, A.G. Meiering, J.D. Cunningham and C. Fyfe. 1982. An integrative approach to the electrophoresis of reductase and dehydrogenase isozymes in respiring and fermenting yeasts. Microbiology 33: 111–118.
Novick, A. and L. Szilard. 1950. Experiments with the chemostat on spontaneous mutations of bacteria. Proc. Natl. Acad. Sci. USA 36: 708–719.
Ohara, Y. and H. Nonomura. 1956. Dynamic aspect of yeast-flora during vinous fermentation. 2. Identification and enological properties of isolates. J. Agric. Chem. Soc. Japan 30: 524–528.
Powell, J.R. 1975. Isozymes and non-Darwinian evolution: a re-evalution. In: Isozymes, Vol. IV, Genetics and Evolution (Markert, C.L., ed.) Academic Press, New York.
Ridgeway, G.J., S.W. Sherburne and R.D. Lewis. 1970. Polymorphisms in the esterases of Atlantic herring. Trans. Am. Fish. Soc. 99: 147–151.
Righelato, R.C., D. Rose and A.W. Westwood. 1981. Kinetics of ethanol production by yeast in continuous culture. Biotechnol. Lett. 3(1): 3–8.
Rosini, G. 1984. Assessment of dominance of added yeast in wine fermentation and origin ofSaccharomyces cerevisiae in wine making. J. Gen. Appl. Microbiol. 30: 249–256.
Russell, I. and G.G. Stewart. 1981. Liquid nitrogen storage of yeast cultures compared to more traditional storage methods. Am. Soc. Brew. Chem. J. 39(1): 19–24.
Scribailo, R.W., K. Carey and U. Posluszny. 1984. Isozyme variation and the reproductive biology ofHydrocharis morsus — ranae L. (Hydrocharitaceae). Bot. J. Linn. Soc. 89: 305–312.
Siciliano, M.J. and C.R. Shaw. 1976. Separation and visualization of enzymes on gels. In: Chromatographic and electrophoretic techniques (Smith, I. ed.), Vol. 2, pp. 185–209, Heinemann, London.
Snow, R. 1979. Toward genetic improvement of wine yeast. Am. J. Enol. Vitic. 30(1): 33–37.
Stewart, G.G. 1981. The genetic manipulation of industrial yeast strains. Can. J. Microb. 27: 973–990.
Subden, R.E. 1983. Wine Yeasts. Dev. Ind. Microbiol. 24: 221–229.
Subden, R.E., D. Irwin, J.D. Cunningham and A.G. Meiering. 1982. Wine yeast isozymes. I. Genetic differences in eighteen stock cultures. Can. J. Microbiol. 28: 1047–1050.
Valade, M. and J. Moulin. 1984. Le levurage en vinification en blanc. Rev. Fr. Oenol. 24(93): 43–56.
Wellman, A.M. and G.G. Stewart. 1973. Storage of brewing yeast by liquid nitrogen refrigeration. Appl. Microbiol. 26(4): 577–583.
Williams, S.A., R.A. Hodges, T.L. Strike R. Snow and R.E. Kunkee. 1984. Cloning the gene for the malo-lactic fermentation of wine fromLactobacillus delbruekii inEscherichia coli and yeast. Appl. Environ. Microbiol. 47: 288–293.
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Subden, R.E., Charlebois, R.L. & Carey, C.K. Selection in yeast I: Assessing genetic stability and relative fitness of commercial yeasts. Journal of Industrial Microbiology 2, 159–165 (1987). https://doi.org/10.1007/BF01569423
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DOI: https://doi.org/10.1007/BF01569423