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DNA-DNA hybridizations among lactic streptococcal temperate and virulent phages belonging to distinct lytic groups

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Journal of Industrial Microbiology

Summary

Ten lactic streptococcal temperate phages and eight lactic streptococcal virulent phages classified on the basis of host range were differentiated by DNA-DNA hybridization. Virulent phages were classified in two distinct homology groups and temperate phages in a single one. In both temperate and virulent phages, no correlation was found between DNA homology groups and lytic groups. For most of the virulent phages, no DNA-DNA hybridization occurred with the temperature phages; however, partial sequence homology was found with DNAs from two virulent phages and four temperate phages.

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References

  1. Adams, M.H. 1959. Bacteriophages, pp. 454–456, Interscience Publishers, Inc., New York.

    Google Scholar 

  2. Budde-Niekel, A., V. Möller, J. Lembke and M. Teuber. 1985. Ökologie von bakteriophagen in einer frischkäserei. Milchwissenschaft 40: 477–481.

    Google Scholar 

  3. Chopin, M.-C., A. Chopin and C. Roux. 1976. Definition of bacteriophage groups according to their lytic action on mesophilic lactic streptococci. Appl. Environ. Microbiol. 32: 741–746.

    Google Scholar 

  4. Daly, C. and G.F. Fitzgerald. 1982. Bacteriophage DNA restriction and the lactic streptococci. In: Microbiology 1982 (Schlessinger, D., ed.), pp. 213–216, American Society for Microbiology, Washington, DC.

    Google Scholar 

  5. Hintermann, G., H.M. Fischer, R. Crameri and R. Hutter. 1981. Simple procedure for distinguishing CCC, OC, and L forms of plasmid DNA by agarose gel electrophoresis. Plasmid 5: 371–373.

    PubMed  Google Scholar 

  6. Huggins, A.R. and W.E. Sandine. 1977. Incidence and properties of temperate bacteriophages induced from lactic streptococci. Appl. Environ. Microbiol. 33: 184–191.

    PubMed  Google Scholar 

  7. Jarvis, A.W. 1984. DNA-DNA homology between lactic streptococci and their temperate and lytic phages. Appl. Environ. Microbiol. 47: 1031–1038.

    Google Scholar 

  8. Jarvis, A.W. and J. Meyer. 1986. Electron microscopic heteroduplex study and restriction endonuclease cleavage analysis of the DNA genomes of three lactic streptococcal phages. Appl. Environ. Microbiol. 51: 566–576.

    PubMed  Google Scholar 

  9. Lawrence, R.C., T.D. Thomas and B.E. Terzaghi. 1976. Reviews of the progress of dairy science: cheese starters. J. Dairy Res. 43: 141–193.

    Google Scholar 

  10. Lowrie, R.J. 1974. Lysogenic strains of group N lactic streptococci. Appl. Microbiol. 27: 210–217.

    PubMed  Google Scholar 

  11. Maniatis, T., E.F. Fritsch and J. Sambrook. 1982. Molecular Cloning: a Laboratory Manual, pp. 85 and 327, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  12. Park, C. and L.L. McKay. 1975. Induction of prophage in lactic streptococci isolated from commercial dairy starter cultures. J. Milk Food Technol. 38: 594–597.

    Google Scholar 

  13. Reyrolle, J., M.-C. Chopin, F. Letellier and G. Novel. 1982. Lysogenic strains of lactic acid streptococci and lytic spectra of their temperate bacteriophages. Appl. Environ. Microbiol. 43: 349–356.

    Google Scholar 

  14. Sanger, F., A.R. Coulson, G.F. Hong, D.F. Hill and G.B. Petersen. 1982. Nucleotide sequence of bacteriophage lambda DNA. J. Mol. Biol. 162: 729–773.

    PubMed  Google Scholar 

  15. Shimizu-Kadota, M., T. Sakurai and N. Tsuchida. 1983. Prophage origin of a virulent phage appearing on fermentations ofLactobacillus casei S-1. Appl. Environ. Microbiol. 45: 669–674.

    Google Scholar 

  16. Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517.

    PubMed  Google Scholar 

  17. Terzaghi, B.E. and W.E. Sandine. 1975. Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 29: 807–813.

    Google Scholar 

  18. Teuber, M. and J. Lembke. 1983. The bacteriophages of lactic acid bacteria with emphasis on genetic aspects of group N streptococci. Antonie van Leeuwenhoek 49: 283–295.

    PubMed  Google Scholar 

  19. Yamamoto, K.R. and B.M. Alberts. 1970. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification. Virology 40: 734–744.

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Génétique Microbienne, Institut de Recherche de Biologie Appliquée (I.R.B.A.), Université de Caen, 14032, Caen, France

    Martine Lautier & Georges Novel

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  1. Martine Lautier
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  2. Georges Novel
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Lautier, M., Novel, G. DNA-DNA hybridizations among lactic streptococcal temperate and virulent phages belonging to distinct lytic groups. Journal of Industrial Microbiology 2, 151–158 (1987). https://doi.org/10.1007/BF01569422

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  • DOI: https://doi.org/10.1007/BF01569422

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