Summary
Transformation experiments using pRO1825 containing the tetracycline resistance transposonTn1721 were conducted to induce mutations in plasmid pSRQ50, which coded for acyclic isoprenol degradation. Transformants that were tetracycline resistant were examined for their ability to utilize geraniol. Strains unable to utilize geraniol were observed to have a 0.6 kb addition on the C fragment of pSRQ50.
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Vandenbergh, P.A., Cole, R.L. Tn1721-induced mutation in an isoprenol degrading plasmid fromPseudomonas putida . Journal of Industrial Microbiology 1, 271–274 (1986). https://doi.org/10.1007/BF01569282
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DOI: https://doi.org/10.1007/BF01569282