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Measure ofLegionella pneumophila activity in situ

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Abstract

Detection ofLegionella pneumophila by serogroup-specific fluorescent antibodies was combined with a tetrazolium dye (INT) to measure electron transport activity. The biological uptake and reduction of the INT dye was studied in pure cultures and in natural water samples with respect to temperature. Uptake was complete within 60 min. Controls inhibited with formaldehyde demonstrated little activity. Both the in vitro and in situ determinations suggested that the electron transport system ofLegionella was active over a temperature range of 25 to 60°C.

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Authors and Affiliations

  1. E. I. du Pont de Nemours & Co., Savannah River Laboratory, 29808, Aiken, South Carolina, USA

    Carl B. Fliermans

  2. Rensselaer Polytechnic Institute, 12181, Troy, New York, USA

    Reginald J. Soracco & Daniel H. Pope

Authors
  1. Carl B. Fliermans
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  2. Reginald J. Soracco
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  3. Daniel H. Pope
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Fliermans, C.B., Soracco, R.J. & Pope, D.H. Measure ofLegionella pneumophila activity in situ. Current Microbiology 6, 89–94 (1981). https://doi.org/10.1007/BF01569010

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