Skip to main content
SpringerLink
Log in

Differentiation of acidophilic thiobacilli by cell density in renografin gradients

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Thiobacillus acidophilus andT. ferrooxidans were separated by centrifugation on the basis of their cell density in Renografin gradients. For both species, growth history was the largest factor influencing cell density. Density was greatest forT. acidophilus andT. ferrooxidans grown with tetrathionate, followed byT. acidophilus grown with glucose.T. ferrooxidans grown with ferrous sulfate was the least dense.T. acidophilus was isolated from iron-grownT. ferrooxidans by separation in a Renografin gradient. Plasmid patterns ofT. acidophilus andT. ferrooxidans were used to confirm the separation of the two species in mixed gradients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Arkensteyn, G. J. M. W., de Bont, J. A. M. 1980.Thiobacillus acidophilus: A study of its presence inThiobacillus ferrooxidans cultures. Canadian Journal of Microbiology26:1057–1065.

    PubMed  Google Scholar 

  2. Cahn, F. H., Fox, M. S. 1968. Fractionation of transformable bacteria from competent cultures ofBacillus subtilis on Renografin gradients. Journal of Bacteriology95:867–875.

    PubMed  Google Scholar 

  3. Dawes, I. W., Wright, J. F., Vezinhet, F., Ajam, N. 1980. Separation on Urografin gradients of subpopulations from sporulatingSaccharomyces cerevisiae cultures. Journal of General Microbiology119:165–171.

    Google Scholar 

  4. Dean, D. H., Douthit, H. D. 1974. Buoyant density heterogeneity in spores ofBacillus subtilis: Biochemical and physiological basis. Journal of Bacteriology117:601–610.

    PubMed  Google Scholar 

  5. Guay, R., Silver, M. 1975.Thiobacillus acidophilus sp. nov.; isolation and some physiological characteristics. Canadian Journal of Microbiology21:281–288.

    PubMed  Google Scholar 

  6. Guay, R., Silver, M., Torma, A. E. 1976. Base composition of DNA isolated fromThiobacillus ferrooxidans grown on different substrates. Revue Canadienne de Biologie35:61–67.

    PubMed  Google Scholar 

  7. Hadden, C., Nester, E. W. 1968. Purification of competent cells in theBacillus subtilis transformation system. Journal of Bacteriology95:876–885.

    PubMed  Google Scholar 

  8. Harrison, A. P., Jarvis, B. W., Johnson, J. L. 1980. Heterotrophic bacteria from cultures of autotrophicThiobacillus ferrooxidans: Relationships as studied by means of deoxyribonucleic acid homology. Journal of Bacteriology143:448–454.

    PubMed  Google Scholar 

  9. Mao, M. W. H., Dugan, P. R., Martin, P. A. W., Tuovinen, O. H. 1980. Plasmid DNA in chemoorganotrophicThiobacillus ferrooxidans andT. acidophilus. FEMS Microbiology Letters8:121–125.

    Google Scholar 

  10. Martin, P. A. W., Dugan, P. R., Tuovinen, O. H. 1981. Plasmid DNA in acidophilic, chemolithotrophic thiobacilli. Canadian Journal of Microbiology27:850–853.

    PubMed  Google Scholar 

  11. Prentice, G. A., Wolfe, F. H., Clegg, L. F. L. 1972. The use of density gradient centrifugation for the separation of germinated from non-germinated spores. Journal of Applied Bacteriology35:345–349.

    PubMed  Google Scholar 

  12. Reed, W. M. R., Dugan, P. R. 1979. Study of developmental stages ofMethylosinus trichoporium with the aid of fluorescent-antibody staining techniques. Applied and Environmental Microbiology38:1179–1183.

    Google Scholar 

  13. Schatz, G., Halsbrunner, E., Tuppy, H. 1964. Deoxyribonucleic acid associated with yeast mitochondria. Biochemical and Biophysical Research Communications15:127–132.

    Google Scholar 

  14. Shafia, F., Brinson, K. R., Heinzman, M. W., Brady, J. M. 1972. Transition of chemolithotrophFerrobacillus ferrooxidans to obligate organotrophy and metabolic capabilities of glucose-grown cells. Journal of Bacteriology111:56–65.

    PubMed  Google Scholar 

  15. Silver, M. 1978. Metabolic mechanisms in iron-oxidizing thiobacilli, pp. 3–17 In: Murr, L. E., Torma, A. E., Brierley, J. A. (eds.), Metallurgical applications of bacterial leaching and related microbiological phenomena. New York: Academic Press.

    Google Scholar 

  16. Tamir, J., Gilvarg, C. 1966. Density gradient centrifugation for the separation of sporulating forms of bacteria. Journal of Biological Chemistry241:1085–1090.

    PubMed  Google Scholar 

  17. Tuovinen, O. H., Kelly, D. P. 1973. Studies on the growth ofThiobacillus ferrooxidans. I. Use of membrane filters and ferrous iron agar to determine viable numbers, and comparison with14CO2-fixation and iron oxidation as measures of growth. Archiv für Mikrobiologie88:285–298.

    Google Scholar 

  18. Tuovinen, O. H., Kelly, D. P. 1974. Studies on the growth ofThiobacillus ferrooxidans. V. Factors affecting growth in liquid culture and development of colonies on solid media containing inorganic sulphur compounds. Archives of Microbiology98:351–364.

    PubMed  Google Scholar 

  19. Tuovinen, O. H., Kelly, D. P., Dow, C. S., Eccleston, M. 1978. Metabolic transitions in cultures of acidophilic thiobacilli, pp. 61–81. In: Murr, L. E., Torma, A. E., Brierley, J. A. (eds.), Metallurgical applications of bacterial leaching and related microbiological phenomena. New York: Academic Press.

    Google Scholar 

  20. Wood, A. P., Kelly, D. P. 1978. Comparative radiorespirometric studies of glucose oxidation in three facultatively heterotrophic thiobacilli. FEMS Microbiology Letters4:283–286.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Insect Pathology Laboratory, U. S. Department of Agriculture, 20705, Beltsville, Maryland, USA

    Phyllis A. W. Martin

  2. Department of Microbiology, The Ohio State University, 43210, Columbus, Ohio, USA

    Patrick R. Dugan & Olli H. Tuovinen

Authors
  1. Phyllis A. W. Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patrick R. Dugan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Olli H. Tuovinen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Martin, P.A.W., Dugan, P.R. & Tuovinen, O.H. Differentiation of acidophilic thiobacilli by cell density in renografin gradients. Current Microbiology 6, 81–84 (1981). https://doi.org/10.1007/BF01569008

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01569008

Keywords

Search

Navigation