Abstract
Erwinia carotovora metabolizes galactose by the Leloir pathway. The synthesis of galactokinase, UDP galactose-4-epimerase, and galactose-1-phosphate uridyl transferase was inducible and under catabolite repression, whereas UDP glucose-pyrophosphorylase was constitutive. Mud1 mutagenesis ofErwinia carotovora resulted in agalU-lac fusion that showed constitutive synthesis ofβ-galactosidase.
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Literature Cited
Adhya SL, Shapiro JA (1969) The galactose operon ofE. coli. I. Structural and pleiotropic mutations of the operon. Genetics 62:231–247
Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Buttin G (1963) Mecanismes regulateurs dans le biosynthese des enzymes du metabolisme du galactose chezE. coli K-12. II. Le determinisme genetique de la regulation. J Mol Biol 7:183–205
Chatterjee AK, Starr MP (1980) Genetics ofErwinia species. Annu Rev Microbiol 34:645–676
Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J (1983)β-Galactosidase gene fusion for analysing gene expression inEscherichia coli and yeast. Methods Enzymol 100:293–308
Csonka LN, Howe MM, Ingraham JL, Pierson III LS, Turnbough CL Jr (1981) Infection ofSalmonella typhimurium with coliphage Mud1 (Apr lac): construction ofpyr::lac gene fusions. J Bacteriol 145:299–305
Diolez A, Coleno A (1985) Mu-lac insertion directed mutagenesis in a pectate lyase gene ofErwinia chrysanthemi. J Bacteriol 163:913–917
Expert D, Toussaint A (1985) Bactericin-resistant mutant ofErwinia chrysanthemi: possible involvement of iron aquisition in phytophathogenicity. J Bacteriol 163:221–227
Forbes KJ, Perombelon MCM (1985) Chromosomal mapping inErwinia carotovora subsp.carotovora with the IncP plasmid R68::Mu. J Bacteriol 164:1110–1116
Hansen RG, Albrecht GJ, Bas ST, Seifert LL (1966) UDP glucose pyrophosphorylase (crystalline) from liver. Methods Enzymol 8:248–253
Hinton JCD, Perombelon MCM, Salmond GPC (1985) Efficient transformation ofErwinia carotovora subsp.carotovora andE. carotovora subsp.atroseptica. J Bacteriol 161:786–788
Hogouvieu-Cotte-Pattat N, Robert-Baudouy J (1985) Isolation ofkdgK-lac andkdgA-lac gene fusions in the phytopathogenic bacteriumErwinia chrysanthemi. J Gen Microbiol 131:1205–1211
Hogouvieux-Cotte-Pattat N, Robert-Baudouy J (1985) The lactose metabolism ofErwinia chrysanthemi. J Bacteriol 162:248–255
Jayaswal RK (1985) Molecular approach to study the pathogenicity ofErwinia carotovora subsp.carotovora. PhD thesis, Purdue University, West Lafayette, Indiana
Jayaswal RK, Bressan RA, Handa AK (1984) Mutagenesis ofE. carotovora subsp.carotovora with bacteriophage Mud1 (Apr lac cts62): construction ofhis-lac gene fusions. J Bacteriol 158:764–766
Jayaswal RK, Bressan RA, Handa AK (1985) Effects of a mutation that eliminates UDP glucose-pyrophosphorylase on the pathogenicity ofErwinia carotovora subsp.carotovora. J Bacteriol 164:473–476
Jayaswal RK, Bressan RA, Handa AK (1985) Behavior of bacteriophage P1 inErwinia carotovora subsp.carotovora. Curr Microbiol 12:73–78
Joseph E, Danchin A, Ullmann A (1981) Regulation of galactose operon expression: glucose effect and role of cyclic adenosine 3′, 5′-monophosphate. J Bacteriol 146:149–154
Kalckar HM, Kurahashi KK, Jordan F (1959) Hereditary defects of galactose metabolism inEscherichia coli. I. Determination of enzyme activities. Proc Natl Acad Sci USA 45:1776–1780
Kilgore WW, Starr MP (1959) Catabolism of galacturonic acid and glucuronic acid s byErwinia carotovora. J Biol Chem 234:2227–2235
Lund BM (1971) Bacterial spoilage of vegetables on certain fruits. J Appl Bacteriol 34:9–20
MacNeil D, Zhu J, Brill W (1981) Regulation of nitrogen fixation inKlebsiella pneumoniae: isolation and characterization of strains withnif-lac fusions. J Bacteriol 145:348
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor NY: Cold Spring Harbor Laboratory
Olson Eric R, Sandowsky MJ, Verma DPS (1985) Identification of genes involved in theRhizobium-legume symbiosis by Mud1 (Kan,lac)-generated transcription fusions. Biotechnology 3:143–149
Perombelon MCM, Boucher C (1979) Developing a mating system inErwinia carotovora. In: Proceedings of the 4th international conference on plant pathogenic bacteria. Beaucouze, France: Angers, pp 47–52
Starr MP, Chatterjee AK (1972) The genusErwinia: enterobacteria pathogenic to plants and animals. Annu Rev Microbiol 26:389–426
Sundarajan TA, Rapin AMC, Kalckar HM (1962) Biochemical observations onE. coli mutants defective in uridine diphosphoglucose. Proc Natl Acad Sci USA 48:2187–2193
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Jayaswal, R.K. Galactose metabolism inErwinia carotovora Subsp.carotovora . Current Microbiology 13, 227–230 (1986). https://doi.org/10.1007/BF01568951
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DOI: https://doi.org/10.1007/BF01568951