Abstract
The protoplasting and transformation techniques described by Chang and Cohen [5] have been modified by the inclusion of mutanolysin and these techniques have been used to prepare protoplasts of a number ofBacillus spp. Cells of some, however, remained resistant to cell wall hydrolysis by both mutanolysin and lysozyme. Protoplasts were prepared from sixBacillus species, including a strain ofB. subtilis, and transformed with the plasmid pUB110. Transformation with the shuttle vector pHV33 was, however, less successful and antibiotic-resistant protoplasts, although detected, either failed to regenerate their osmotic stability or rapidly lost their antibiotic resistance.
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Mann, S.P., Orpin, C.G. & Hazlewood, G.P. Transformation ofBacillus spp.: An examination of the transformation ofBacillus protoplasts by plasmids pUB110 and pHV33. Current Microbiology 13, 191–195 (1986). https://doi.org/10.1007/BF01568945
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DOI: https://doi.org/10.1007/BF01568945