Abstract
J-774 murine macrophages were allowed to multiply in the presence of gammairradiated (4.5×106 rads)Mycobacterium avium for 3 days. The macrophages thus stimulated and still containing killed bacteria were then challenged with viableM. avium bacteria, and the intracellular growth of these bacilli was measured during 1 week by lysing the J-774 cells and measuring the viable bacterial counts on 7H10 ager. The results were compared with those obtained in parallel with normal J-774 cells. Our results showed that pretreatment of macrophages with killedM. avium neither enhanced their capacity to check the intracellular growth of viableM. avium nor did it potentiate the intracellular activity of rifampicin, ansamycin and clofazimine against actively multiplyingM. avium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Banerjee DK, Sharp AK, Lowrie DB (1986) The effect of gamma interferon duringMycobacterium bovis (BCG) infection in athymic and euthymic mice. Microb Pathogen 1:221–224
Crowle AJ, Ross EJ, May MH (1987) Inhibition by 1,25-(OH)2 vitamin D3 of the multiplication of virulent tubercle bacilli in cultured human macrophages. Infect Immun 55:2945–2950
Ivanyi J (1986) Pathogenic and protective interactions in mycobacteria. Clin Immunol Aller 6:127–157
Lowrie DB (1983) How macrophages kill tubercle bacilli. Tubercle 16:1–12
Lowrie DB (1983) Mononuclear phagocyte-mycobacterium interaction. In: Ratledge C, Stanford JO (eds) The biology of the mycobacteria, vol. 2. London: Academic Press, pp 235–278
Nagao S, Miki T, Tanak A (1981) Macrophage activation by muramyl dipeptide (MDP) without lymphocyte participation. Curr Topics Microbiol Immunol 25:41–50
Poulter LW, Rook GA, Steele J, Condez A (1987) Influence of 1,25-(OH)2 vitamin D3 and gamma interferon on the phenotype of human peripheral blood monocyte-derived macrophages. Infect Immun 55:2017–2020
Rastogi N, Potar M-C, David HL (1987) Intracellular growth of pathogenic mycobacteria in the continuous murine macrophage cell line J-774: ultrastructure and drug-susceptibility studies. Curr Microbiol 16:79–92
Rook GAW, Steele J, Ainsworth M, Champion BR (1986) Activation of macrophages to inhibit proliferation ofMycobacterium tuberculosis: comparison of the effects of recombinant gamma-interferon on human monocytes and murine peritoneal macrophages. Immunology 59:333–338
Saito H, Tomioka H, Yamada Y, Jidoi J (1986) Oxidative and phagocytic functions of macrophages during infections induced in mice byMycobacterium intracellulare andListeria monocytogenes. J Gen Microbiol 132:1117–1125
Sharp AK, Banerjee DK (1986) Effect of gamma interferon on hydrogen peroxide production by cultured mouse peritoneal macrophages. Infect Immun 54:597–599
Steele J, Flint KC, Pozniak AL, Hudspith B, Johnson MM, Rook GAW (1986) Inhibition of virulentMycobacterium tuberculosis by murine peritoneal macrophages and human alveolar lavage cells: the effects of lymphokines and recombinant gamma-interferon. Tubercle 67:289–294
Wadee AA, Cohen JD, Rabson AR (1987) Gamma-interferon reverses inhibition of leukocyte bactericidal activity by a 25-kilodalton fraction fromMycobacterium tuberculosis. Infect Immun 55:2777–2782
Zlotnik A, Crowle AJ (1982) Lymphokine-induced mycobacteriostatic activity in mouse pleural macrophages. Infect Immun 37:786–793
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rastogi, N., Potar, MC. & David, H.L. Prolonged exposure of J-774 macrophages to gamma-killedMycobacterium avium did not affect their inability to check the intracellular growth of viableM. avium . Current Microbiology 18, 23–25 (1989). https://doi.org/10.1007/BF01568825
Issue Date:
DOI: https://doi.org/10.1007/BF01568825