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Protein alterations in isolated outer membranes of polymyxin-resistantPseudomonas aeruginosa isolates

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Abstract

Isolated outer membranes fromPseudomonas aeruginosa strains resistant to polymyxin were compared with isolated outer membranes from polymyxin-sensitive strains as to their protein composition as determined by slab polyacrylamide gel electrophoresis. Both the porin protein and the H1 protein were decreased greatly in concentration in the outer membranes from the polymyxin-resistant strains. This confirms previous findings reduced concentrations of these proteins in cell envelopes of these strains. No evidence was found for these decreases being the result of an artifactual loss of these proteins from the outer membrane due to conditions used for growth or for preparation of cell envelopes. Nevertheless, the role of these outer membrane proteins in mediating polymyxin resistance is uncertain.

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Literature Cited

  1. Annear, D. I. 1976. Linked and unstable characteristics of colony structure, gentamicin resistance and colistin resistance inPseudomonas aeruginosa. Microbios Letters3:203–206.

    Google Scholar 

  2. Brown, M. R. W., Watkins, W. M. 1970. Low magnesium and phospholipid content of cell walls ofPseudomonas aeruginosa resistant to polymyxin. Nature (London)227:1360–1361.

    Google Scholar 

  3. Conrad, R. S., Gilleland, H. E., Jr. 1981. Lipid alterations in cell envelopes of polymyxin-resistantPseudomonas aeruginosa isolates. Journal of Bacteriology148:487–497.

    PubMed  Google Scholar 

  4. Conrad, R. S., Wulf, R. G., Ankrom, K. A. 1981. Fatty acids ofPseudomonas aeruginosa grown on media affecting polymyxin susceptibility. Current Microbiology5:231–234.

    Google Scholar 

  5. Conrad, R. S., Wulf, R. G., Clay, D. L. 1979. Effects of carbon sources on antibiotic resistance inPseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy15:59–66.

    PubMed  Google Scholar 

  6. Gilleland, H. E., Jr. 1977. Ultrastructural alteration of the outer membrane ofPseudomonas aeruginosa associated with resistance to polymyxin B and to EDTA, pp. 145–150. In: Schlessinger, D. (ed.), Microbiology—1977. Washington, D.C.: American Society for Microbiology.

    Google Scholar 

  7. Gilleland, H. E., Jr., Conrad, R. S. 1980. Effects of carbon sources on chemical composition of cell envelopes ofPseudomonas aeruginosa in association with polymyxin resistance. Antimicrobial Agents and Chemotherapy17:623–628.

    PubMed  Google Scholar 

  8. Gilleland, H. E., Jr., Lyle, R. D. 1979. Chemical alterations in cell envelopes of polymyxin-resistantPseudomonas aeruginosa isolates. Journal of Bacteriology138:839–845.

    PubMed  Google Scholar 

  9. Gilleland, H. E., Jr., Murray, R. G. E. 1976. Ultrastructural study of polymyxin-resistant isolates ofPseudomonas aeruginosa. Journal of Bacteriology125:267–281.

    PubMed  Google Scholar 

  10. Gilleland, H. E. Jr., Stinnett, J. D., Eagon, R. G. 1974. Ultrastructural and chemical alteration of the cell envelope ofPseudomonas aeruginosa, associated with resistance to ethylenediaminetetraacetate resulting from growth in Mg2+-deficient medium. Journal of Bacteriology117:302–311.

    PubMed  Google Scholar 

  11. Greenwood, D. 1975. The activity of polymyxins against dense populations ofEscherichia coli. Journal of General Microbiology91:110–118.

    PubMed  Google Scholar 

  12. Hancock, R. E. W., Carey, A. M. 1979. Outer membrane ofPseudomonas aeruginosa: heat- and 2-mercaptoethanolmodifiable proteins. Journal of Bacteriology140:902–910.

    PubMed  Google Scholar 

  13. Hancock, R. E. W., Decad, G. M., Nikaido, H. 1979. Identification of the protein producing transmembrane diffusion pores in the outer membrane ofPseudomonas aeruginosa PAO1. Biochimica et Biophysica Acta554:323–331.

    PubMed  Google Scholar 

  14. Hancock, R. E. W., Raffle, V. J., Nicas, T. I. 1981. Involvement of the outer membrane in gentamicin and streptomycin uptake and killing inPseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy19:777–785.

    PubMed  Google Scholar 

  15. Harder, K. J., Nikaido, H., Matsuhashi, M. 1981. Mutants ofEscherichia coli that are resistant to certain beta-lactam compounds lack theompF porin. Antimicrobial Agents and Chemotherapy20:549–552.

    PubMed  Google Scholar 

  16. Nicas, T. I., Hancock, R. E. W. 1980. Outer membrane protein H1 ofPseudomonas aeruginosa: Involvement in adaptive and mutational resistance to ethylenediaminetetraacetate, polymyxin B, and gentamicin. Journal of Bacteriology143:872–878.

    PubMed  Google Scholar 

  17. Shimizu, S., Iyobe, S., Mitsuhashi, S. 1977. Inducible high resistance to colistin inProteus strains. Antimicrobial Agents and Chemotherapy12:1–3.

    PubMed  Google Scholar 

  18. Stan-Lotter, H., Sanderson, K. E. 1981. Interaction of cations with membrane fractions of smooth and rough strains ofSalmonella typhimurium and other gram-negative bacteria. Journal of Bacteriology146:542–551.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Louisiana State University Medical Center, School of Medicine in Shreveport, 71130, Shreveport, Louisiana, USA

    Harry E. Gilleland Jr. & Marion W. Beckham

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  1. Harry E. Gilleland Jr.
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  2. Marion W. Beckham
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Gilleland, H.E., Beckham, M.W. Protein alterations in isolated outer membranes of polymyxin-resistantPseudomonas aeruginosa isolates. Current Microbiology 7, 235–239 (1982). https://doi.org/10.1007/BF01568805

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