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Phosphomannomutase activity in wild-type and alginate-producing strains ofPseudomonas aeruginosa

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Abstract

Phosphomannomutase (PMM) activity was detected in the soluble cytoplasmic fraction of crude extracts of both mucoid (alginate-producing) and nonmucoid strains ofPseudomonas aeruginosa. The enzyme activity was concentrated and partially purified from cell extracts of mucoid strain V388 by precipitation with ammonium sulfate and by molecular exclusion chromatography. These preparations catalyzed the conversion of mannose 1-phosphate to mannose 6-phosphate in a coupled assay system that contained commercial phosphomannoisomerase, phosphoglucoisomerase, and glucose 6-phosphate dehydrogenase. Catalytic activity in this system was strictly dependent on the presence of glucose 1,6-diphosphate (apparent Km, 150 μM) and exhibited a pH optimum of around 9 in Bicine-NaOH buffer. PMM exhibited an apparent Km of 60 μM for mannose 1-phosphate, but concentrations greater than 150 μM caused significant inhibition. Specific activities of PMM were consistently higher in the soluble fractions of mucoid strains (1.2–3.6 nmol/min/mg protein) than of nonmucoid strains (0.2–0.6 nmol/min/mg protein).

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Author notes

  1. Penelope J. Padgett

    Present address: Department of Biology, Shippensburg University, 17257, Shippensburg, PA, USA

  2. Paul V. Phibbs Jr.

    Present address: Department of Microbiology and Immunology, School of Medicine, East Carolina University, 27834-4354, Greenville, NC, USA

Authors and Affiliations

  1. Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia, USA

    Penelope J. Padgett & Paul V. Phibbs Jr.

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  1. Penelope J. Padgett
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  2. Paul V. Phibbs Jr.
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Padgett, P.J., Phibbs, P.V. Phosphomannomutase activity in wild-type and alginate-producing strains ofPseudomonas aeruginosa . Current Microbiology 14, 187–192 (1986). https://doi.org/10.1007/BF01568516

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