Abstract
The fermentation ofd-arabinose byBacteroides ruminicola strain B14 occurs in a manner similar to or identical with that shown previously forl-arabinose metabolism by the organism, a combination of hexose resynthesis and the Embden-Meyerhof sequence. The use ofd-arabinose by strain B14 was repressed by prior growth in medium containingd-glucose and induced by prior growth in the presence ofl-arabinose ord-xylose. The use ofd-ribose andd-xylose by strain B14 is different from that ford-arabinose. During growth in the presence of 1-14C-d-arabinose, labeled acetate, propionate, and succinate were formed, whereas during 1-14C-d-ribose growth only labeled acetate and propionate were obtained. Under the conditions used,d-xylose growth failed to allow formation of acetate, propionate, or succinate. Strain B14 incorporates label from 1- or 2-labeled glycine into acetate, propionate, and succinate by a mechanism involving the cleavage of glycine and equilibration of glycine carbons 1 and 2 with different metabolic pools.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Bradford M (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Bruno CF, Moore WEC (1962) Fate of lactic acid in rumen digesta. J Dairy Sci 45:109–115
Bryant MP, Robinson IM (1962) Some nutritional characteristics of predominant cultural rumen bacteria. J Bacteriol 82:605–614
Caldwell DR, Newman K (1986)d-Arabinose metabolism byBacteroides fraglis strain 2044. Curr Microbiol 14:55–59
Doelle HW (1975) Bacterial metabolism. New York: Academic Press, pp 274–281
Guthrie RD (1974) Introduction to the chemistry of carbohydrates, 4th edn. Oxford: Clarendon
Hungate RE (1970) A roll tube method for cultivation of strict anaerobes. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 3B, New York: Academic Press, pp 117–132
Macy JM, Probst I (1979) The biology of gastrointestinalBacteroides. Annu Rev Microbiol 33:562–594
Phares EF, Long EV (1954) The complete degradation of C14-labelled succinic acid and succinic anhydride by the Schmidt reaction. J Am Chem Soc 77:2556–2557
Prins RA (1977) Biochemical activities of gut microorganisms. In: Clarke RTJ, Bauchop T (eds) The microbial ecology of the gut. New York: Academic Press, pp 73–183
Salyers AA (1984) Human colonicBacteroides. Annu Rev Microbiol 38:293–313
Salyers AA, Leedle JAZ (1983) Carbohydrate metabolism in the human colon. In: Hentges D (ed) Human intestinal microflora in health and disease. New York: Academic Press, pp 129–144
Scardovi V, Chiappini MG (1966) Rumen bacteriology. V. Carboxylation of phosphoenol pyruvate in some rumen bacterial strains and in the cell free extract of the total rumen flora. Ann Microbiol Enzymol 16:119–127
Turner KW, Roberton AM (1979) Xylose, arabinose and rhamnose fermentation byBacteroides ruminicola. Appl Environ Microbiol 38:7–12
Wallnofer P, Baldwin RL (1966) Pathway of propionate formation inBacteoides ruminicola. J Bacteriol 93:504–505
Wolin MJ, Miller TL (1983) Carbohydrate fermentation. In: Hentges D (ed) Human intestinal microflora in health and disease. New York: Academic Press, pp 147–165
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Caldwell, D.R., Newman, K. Pentose metabolism byBacteroides ruminicola subsp.brevis strain B14. Current Microbiology 14, 149–155 (1986). https://doi.org/10.1007/BF01568367
Issue Date:
DOI: https://doi.org/10.1007/BF01568367