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Enumeration of chlorine-stressed organisms with acridine orange 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (AOINT)

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Abstract

Direct microscopic enumeration ofEnterobacter cloacae with the acridine orange 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride technique (AOINT) was compared with spread plate counts on nonselective media to establish the usefulness of the former technique in the enumeration of chlorine-stressed cells. Results indicate that the techniques are comparable when the organisms are not stressed. However, AOINT is more sensitive than are plate counts in the detection of chlorine-stressed cells.

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Authors and Affiliations

  1. School of Life and Health Sciences, University of Delaware, Newark, Delaware

    Diane S. Herson, Balkumar Marthi & Mary Anne Payer

  2. Department of Biology, Millersville University of Pennsylvania, 17551, Millersville, Pennsylvania, USA

    Katherine H. Baker

Authors
  1. Diane S. Herson
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  2. Balkumar Marthi
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  3. Mary Anne Payer
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  4. Katherine H. Baker
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Herson, D.S., Marthi, B., Payer, M.A. et al. Enumeration of chlorine-stressed organisms with acridine orange 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (AOINT). Current Microbiology 13, 77–80 (1986). https://doi.org/10.1007/BF01568285

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