Abstract
Ultrastructure of “Gordona aurantiaca”* M 296 (8128) was studied after the lead citrate coloration, whereas the cell envelope architecture was investigated by ruthenium red staining for outer wall acidic polysaccharides and the periodic-acid-thiocarbohydrazide-silver-proteinate cytochemical procedure (Thiéry method) for the detection of “α1-2 glycol bond containing polysaccharides.” The ultrastructural morphology of bacteria was distinct from both the mycobacteria and nocardia. The bacilli had a typical gram-positive cell wall that contained a thin, uniformly distributed, polysaccharide outer layer (POL) at its surface. The Thiéry cytochemical method stained only the cytoplasmic membrane, but not the cell wall, a feature that is common to the mycolic acid containing theCorynebacterium-Mycobacterium-Nocardia (CMN) group of organisms. The negative staining of the unfixed preparations of bacilli showed ribbonlike surface structures, common to the CMN group of organisms. The electron-microscopic preparations showed numerous lysing bacilli with bacteriophages indicating that the strain used was lysogenic.
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Rastogi, N., David, H.L. & Frehel, C. Ultrastructure of “Gordona aurantiaca” Tsukamura 1971. Current Microbiology 13, 51–56 (1986). https://doi.org/10.1007/BF01568160
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DOI: https://doi.org/10.1007/BF01568160