Abstract
The ultraviolet (UV)-induced lethality in excision-proficientEscherichia coli strains WP-2 HCR+, B/r HCR+, B/r (λ) HCR+, Blon − HCR+, and WP-2 HCR− is increased when chloroquine (300–500 μg/ml) is added to the postirradiation medium. The degree to which chloroquine enhances the lethality of UV radiation varies for each strain, with strains B/r (λ) and B showing a greater degree of repair inhibition than the other bacterial strains. The D10 (UV showing 10% survival) decreased in B/r (λ) HCR+ strain grown in the presence of 500 μg/ml (dose response of 2.5). InE. coli B, a dose response of 4.0 was obtained in the presence of the same concentration of chloroquine.Escherichia coli B/r, WP-2 HCR+, and WP-2 HCR− strains showed less UV-induced lethality in the presence of chloroquine. The drug also inhibited “liquid holding recovery” (LHR) in irradiated HCR+ strains. These results suggest that chloroquine interferes with the excision repair (HCR function) of UV-induced photoproducts in irradiated bacterial populations.
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Sideropoulos, A.S., Specht, S.M., Katarincic, J. et al. The role of chloroquine supplementation in liquid holding recovery and ultraviolet lethality ofEscherichia coli strains. Current Microbiology 12, 251–255 (1985). https://doi.org/10.1007/BF01567973
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DOI: https://doi.org/10.1007/BF01567973