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Antigen-binding receptors on T cells from long-term MLR. Evidence of binding sites for allogeneic and self-MHC products

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Abstract

Antibody inhibition of radiolabelled stimulator membrane vesicle binding by T blasts activated in the mixed lymphocyte reaction (MLR) was used to identify responder-cell determinants involved in the binding phenomenon. Antisera or monoclonal antibodies against Thy-1, Lyt-1, Lyt-2 and Ly-6 antigens were not inhibitory. However, antibodies against heavy-chain V region (VH) determinants strongly inhibited vesicle binding by both primary and longterm MLR blasts. Anti-Ia (both alloantisera and monoclonal reagents) caused inhibition of antigen binding by primary MLR blasts only. T blasts from long-term MLR lines were neither Ia-positive, nor susceptible to blocking of antigen binding with anti-Ia. However, these cells were capable of specifically absorbing soluble syngeneic Ia material, with the concomitant appearance of vesiclebinding inhibition with anti-Ia sera. Acquisition of syngeneic Ia by T blasts was effectively blocked with the anti-VH reagent. Passively bound self-Ia did not interfere with vesicle binding in the absence of anti-Ia. These results strongly suggest the existance of specific self-Ia acceptor sites closely linked to the receptors for stimulator alloantigens on T cells proliferating in MLR. A receptor model based on these findings is briefly discussed.

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Abbreviations

B10:

C57BL/10

Con A:

concanavalin A

FcR:

Fc receptor

FCS:

fetal calf serum

H:

heavy chain

Ia:

I-region associated antigen

Ig:

immunoglobulin

LPS:

lipopolysaccharide

Lyt:

T-lymphocyte differentiation antigen

MHC:

major histocompatibility complex

MLR:

mixed lymphocyte reaction

PM:

plasma membrane

T:

thymus derived

Tcr:

T-cell receptor

V:

variable region of Ig

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Author notes

  1. Zoltan A. Nagy

    Present address: Department of Immunogenetics, Max Planck Institute for Biology, 7400, Tübingen 1, Germany

Authors and Affiliations

  1. Cancer Research Laboratories, Botterell Hall, Department of Pathology, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    Bruce E. Elliott & Zoltan A. Nagy

  2. Department of F/Konzern/PAI, F. Hoffman-LaRoche and Company, CH-4002, Basel, Switzerland

    Bela J. Takacs

  3. Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

    Yinon Ben-Neriah & David Givol

Authors
  1. Bruce E. Elliott
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  4. Yinon Ben-Neriah
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  5. David Givol
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Elliott, B.E., Nagy, Z.A., Takacs, B.J. et al. Antigen-binding receptors on T cells from long-term MLR. Evidence of binding sites for allogeneic and self-MHC products. Immunogenetics 11, 177–190 (1980). https://doi.org/10.1007/BF01567783

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  • DOI: https://doi.org/10.1007/BF01567783

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