Abstract
Cytotoxic T lymphocytes generated in the course of an HSV-infection of CBA (H-2 k) mice not only lyse syngeneic, virus-infected target cells but also cross-react with noninfected target cells expressing the Dd alloantigen. On the effector cell level, this alloreactivity is mediated by virus-specific CTL's that are restricted to H-2Dk determinants. On the prekiller cell level, the anti-HSV-reactive T cells exhibiting cross-reactivity for Dd alloantigen could be positively selected on H-2d spleen-cell monolayers. After differentiation into cytolytic effector cells, target cells expressing Dd alloantigens and syngeneic HSV-infected target were lysed with equal efficiency. The results imply that the phenomenon of H-2-restricted versus nonrestricted T-cell reactivity is not due to distinct T-cell subsets, but rather is dependent on the antigeneic determinants recognized.
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Bevan, M. J.: Killer cells reactive to altered-self antigens can also be alloreactive.Proc. Natl. Acad. Sci. U.S.A. 74: 2094–2098, 1977
Bevan, M. J. and Fink, J. P.: The influence of thymus H-2 antigens on the specificity of maturating killer and helper cells.Immunol. Rev. 42: 3–19, 1978
Binz, H. and Wigzell, H.: Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants.J. Exp. Med. 142: 1218–1230, 1975
Cantor, H. and Boyse, E. A.: Regulation of cellular and humoral immune responses by T cell subclasses.Cold Spring Harbor Symp. Quant. Biol. 41: 23–62, 1976
Doherty, P. C., Blanden, R. V., and Zinkernagel, R. M.: Specificity of virus-immune effector T cells for H-2K or H-2D compatible interactions: Implications for H-antigen diversity.Transplant. Rev. 29: 89–124, 1976
Finberg, R., Burakoff, S., Cantor, H., and Benacerraf, B.: Biological significance of alloreactivity: T cells stimulated by Sendai virus-coated syngeneic spleen cells specifically lyse allogeneic target cells.Proc. Natl. Acad. Sci. U.S.A. 15: 5145–5149, 1978
Fischer-Lindahl, K. F., Peck, A. B., and Bach, F. H.: Specificity of cell-mediated lympholysis for public and private H-2 determinants.Scand. J. Immunol. 4: 541–553, 1975
Lindahl, K. D. and Wilson, D. B.: Histocompatibility antigen-activated cytotoxic T lymphocytes. II. Estimates of the frequency and specificity of precursors.J. Exp. Med. 154: 508–522, 1977
Miller, R. G., Teh, T. S., Harley, E., and Phillips, R. A.: Quantitative studies of the activation of cytotoxic lymphocyte precursor cells.Immunol. Rev. 55: 38–58, 1977
Peary, D. and Pierce, C. W.: Cell-mediated immune responses in vitro. II. Simultaneous generation of cytotoxic lymphocyte responses to two sets of alloantigens of limited crossreactivity.J. Immunol. 115: 1515–1520, 1975
Pfizenmaier, K., Jung, H., Starzinski-Powitz, A., Röllinghoff, M., and Wagner, H.: The role of T cells in anti-herpes simplex virus immunity. I. Induction of antigen-specific cytotoxic T lymphocytes.J. Immunol. 119: 939–944, 1977 a
Pfizenmaier, K., Starzinski-Powitz, A., Röllinghoff, M., Falke, D., and Wagner, H.: T cell-mediated cytotoxicity against herpes simplex virus-infected target cells.Nature 265: 630–632, 1977 b
Pfizenmaier, K., Jung, H., Kurrle, R., Röllinghoff, M., and Wagner, H.: Antigen, H-2Dd Alloreactivity mediated by Herpes-Simplex-virus specific cytotoxic H-2k lymphocytes is associated with H-2Dk.Immunogenetics 10: 395–404, 1980
Schnagl, H. Y. and Boyle, W.: Specific depletion of alloreactive cytotoxic lymphocyte precursors.Nature 219: 231–233, 1979
Shearer, G. M. and Schmitt-Verhulst, A. M.: Major histocompatibility complex restricted cell-mediated immunity.Adv. Immunol. 25: 55–91, 1977
Simonsen, M.: The clonal selection hypothesis evaluated by grafted cells reactive against their host.Cold Spring Harbor Symp. Quant. Biol. 32: 571–523, 1967
Simpson, E. and Gordon, R. D.: Responsiveness to HY antigen. Ir gene complementation and target cell specificity.Immunol. Rev. 35: 59–75, 1977
Skinner, M. A. and Marbrook, J.: An estimation of the frequency of precursor cells which generate cytotoxic lymphocytes.J. Exp. Med. 143: 1562–1567, 1976
Taswell, C., MacDonald, H. R., and Cerottini, J. C.: Limiting dilution analysis of alloantigen reactive T lymphocytes. II. Effect of cortisone and cyclophosphamide and cytolytic T lymphocyte precursor frequencies in the thymus.Thymus 1: 119–1979
von Boehmer, H., Hengartner, H., Nabholz, M., Lenhardt, W., Schreier, M. H., and Haas, W.: Fine specificity of a continuously growing killer cell clone specific for H-Y antigen.Eur. J. Immunol. 9: 592–597, 1979
Wagner, H. and Röllinghoff, M.: T-T cell interactions during in vitro cytotoxic allograft responses. I. Soluble products from activated Ly 1+ T cells trigger autonomously antigen primed Ly 23+ T cells to cell proliferation and cytotoxic activity.J. Exp. Med. 148: 1523–1538, 1978
Wagner, H., Pfizenmaier, K., and Röllinghoff, M.: The role of the major histocompatibility gene complex in murine cytotoxic T cell responses.Adv. Cancer Res. 31: 77, 1980 a
Wagner, H., Röllinghoff, M., Pfizenmaier, K., Hardt, C., and Johnscher, G.: T-T cell interactions during in vitro cytotoxic T lymphocyte responses. II. Helper factor from activated Lyt 1+ T cells is rate limiting (i) in T cell responses to non immunogenic alloantigen (ii) in thymocyte responses to allogeneic stimulator cells, and (iii) recruits allo- or H-2 restricted CTL precursors from the Lyt 123+ T cell subset.J. Immunol 124: 1058–1067, 1980 b
Watson, J., Gillis, S., Marbrook, J., Mochizuki, D., and Smith, K. A.: Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines.J. Exp. Med. 150: 849–861, 1979
Zinkernagel, R. M. and Doherty, P. C.: MHC restricted cytotoxic T cells: Studies on the biological role of polymorphic major transplantation antigens determing T cell restriction-specificity, function and responsiveness.Adv. Immunol. 27: 51–177, 1979
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Pfizenmaier, K., Stockinger, H., Röllinghoff, M. et al. Herpes-simplex-virus-specific, H-2Dk-restricted T lymphocytes bear receptors for H-2Dd alloantigen. Immunogenetics 11, 169–176 (1980). https://doi.org/10.1007/BF01567782
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DOI: https://doi.org/10.1007/BF01567782