Abstract
The patterns of expression of several types of genetic variation in murine C4 were investigated. Dominance was assessed forS-region-determined differences in molecular weight of the C4 α-chain fromS w7 strains relative to all other strains examined and in C4 hemolytic titers. Codominant expression in F1 hybrids was shown for both of the above variations. Comparison of tryptic peptide profiles of radiolabeled C4β-chains encoded by differentS regions revealed differences in their primary structures. The demonstration of codominant expression for the variant C4 traits examined and the evidence from tryptic peptide maps for haplotype differences in C4 primary structure strongly support the hypothesis that polymorphism of murine C4 is controlled by structural genes which are located in theS region.
Similar content being viewed by others
References
Atkinson, J. P., McGinnis, K., Brown, L. H., Peterein, J., and Shreffler, D. C.: A murine C4 molecule with reduced hemolytic activity.J. Exp. Med. 151: 492–497, 1980a
Atkinson, J. P., McGinnis, K., and Shreffler, D. C.: Development and characterization of a hemolytic assay for mouse C4.J. Immunol. Methods 33: 351–368, 1980b
Bonner, W. J. and Laskey, R. A.: A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.Eur. J. Biochem. 46: 83–88, 1974
Brown, J. L., Kato, K., Silver, J., and Nathenson, S. G.: Notable diversity in peptide composition of murine H-2K and H-2D alloantigens.Biochemistry 13: 3174–3187, 1974
Carroll, M. C. and Capra, J. D.: Studies on murine Ss protein: Demonstration that Ss protein is functionally the fourth component of complement.Proc. Natl. Acad. Sci. U.S.A. 75: 2424–2428, 1978
Carroll, M. C. and Capra, J. D.: Studies on murine Ss protein: Demonstration thatS locus encodes structural gene for fourth component of complement.Proc. Natl. Acad. Sci. U.S.A. 76: 4641–4645, 1979
Curman, B., Ostberg, L., Sandberg, L., Malmheden-Eriksson, I., Stalenheim, G., Rask, L., and Peterson, P. A.:H-2 linked Ss protein is C4 component of complement.Nature 258: 243–245, 1975
Ferreira, A., Nussenzweig, V., and Gigli, I.: Structural and functional differences between theH-2 controlled Ss and Slp proteins.J. Exp. Med. 148: 1186–1197, 1978
Hansen, T. H., Krasteff, T. N., and Shreffler, D. C.: Quantitative variations in the expression of the mouse serum antigen Ss and its sex-limited allotype Slp.Biochem. Genet. 12: 281–293, 1974
Hansen, T. H.: Studies of regulatory and functional aspects of the Ss serum protein in the mouse. Ph. D. Thesis, University of Michigan, Ann Arbor, 1975
Hansen, T. H. and Shreffler, D. C.: Characterization of a constitutive variant of the murine serum protein allotype, Slp.J. Immunol. 117: 1507–1513, 1976
Laskey, R. A., and Mills, A. D.: Quantitative film detection of [3H] and [14C] in polyacrylamide gels by fluorography.Eur. J. Biochem. 56: 335–341, 1975
Meo, T., Krasteff, T., and Shreffler, D. C.: Immunochemical identification of murineH-2 controlled Ss (serum substance) protein through identification of its human homologue as the fourth component of complement.Proc. Natl Acad. Sci. U.S.A. 72: 4536–4560, 1975
Parker, K. L., Roos, M. H., and Shreffler, D. C.: Structural characterization of the murine fourth component of complement and sex-limited protein and their precursors: Evidence for two loci in the S region of theH-2 complex.Proc. Natl. Acad. Sci. U.S.A. 76: 5853–5857, 1979
Passmore, H. C. and Shreffler, D. C.: A sex-limited serum protein variant in the mouse: Inheritance and association with theH-2 region.Biochem. Genet. 4: 351–365, 1970
Passmore, H. C. and Beisel, K. W.: Preparation of antisera for the detection of the Ss protein and the Slp alloantigen.Immunogenetics 4: 393–399, 1977
Roos, M. H., Atkinson, J. P., and Shreffler, D. C.: Molecular characterization of the Ss and Slp (C4) proteins of the mouseH-2 complex: Subunit composition, chain size polymorphism, and an intracellular (Pro-Ss) precursor.J. Immunol. 121: 1106–1115, 1978
Shreffler, D. C.: A serologically detected variant in mouse serum: Further evidence for genetic control by the histocompatibility-2-locus.Genetics 49: 973–978, 1964
Shreffler, D. C. and Owen, R. D.: Serologically detected variant in mouse serum: Inheritance and association with the histocompatibility-2-locus.Genetics 48: 9–25, 1963
Vitetta, E. S. and Capra, J. D.: The protein products of the murine 17th chromosome: Genetics and structure.Adv. Immunol. 26: 147–193, 1978
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Parker, K.L., Atkinson, J.P., Roos, M.H. et al. Genetic and structural characterization ofH-2-controlled allelic forms of murine C4. Immunogenetics 11, 55–63 (1980). https://doi.org/10.1007/BF01567769
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01567769