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Subcellular distribution of glycoside hydrolase and polysaccharide depolymerase enzymes in the rumen entodiniomorphid ciliatePolyplastron multivesiculatum

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Abstract

The distribution of 12 acid hydrolase and two polysaccharide depolymerase enzymes in the rumen entodiniomorphid ciliatePolyplastron multivesiculatum, isolated from the ovine rumen 2 h after feeding, was examined by differential and density-gradient centrifugation. Approximately 60%–70% of the recovered activity was sedimentable in fractions prepared by centrifugation at 103 g for 10 min (F1) and 104 g for 10 min (F2) with 25%–35% of the acid hydrolases and 15%–20% of acid phosphatase and the polysaccharidases remaining nonsedimentable (in fraction F5) after centrifugation at 105 g for 60 min. Approximately 60% of the sedimentable activity was located in fraction F1. Latency of the hydrolase activity was demonstrated. After isopycnic centrifugation in sucrose density gradients, the hydrolytic enzymes cosedimented in acid phosphatase-containing, membrane-bound, pleomorphic lysosomelike vesicles 0.1–1.0 μm in size, with a mean equilibrium density of 1.17 (1.15–1.19) g/ml.

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Authors and Affiliations

  1. Department of Animal Nutrition and Production, Hannah Research Institute, KA6 5HL, Ayr, Scotland

    Alan G. Williams

  2. Department of Microbiology, University of Glasgow, Glasgow, Scotland

    Alec B. Ellis

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  1. Alan G. Williams
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  2. Alec B. Ellis
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Williams, A.G., Ellis, A.B. Subcellular distribution of glycoside hydrolase and polysaccharide depolymerase enzymes in the rumen entodiniomorphid ciliatePolyplastron multivesiculatum . Current Microbiology 12, 175–181 (1985). https://doi.org/10.1007/BF01567672

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