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Inhibition of translation by the fungal toxin cyclopiazonic acid

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Abstract

Cyclopiazonic acid is a toxic metabolite of fungal origin that inhibits protein synthesis in intact HeLa cells and eukaryotic cell-free systems. It has been shown that cyclopiazonic acid blocks the GTP- and EF-1-dependent binding of (3H)Phe-tRNA to 80S ribosomes. Moreover the translocation of N-Ac-(3H)Phe-tRNA by 80S ribosomes that takes place in the presence of EF-2 and GTP is also halted by cyclopiazonic acid. It is concluded that this drug affects a ribosomal site involved in the alternative interaction of elongation factors EF-1 and EF-2.

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Literature Cited

  1. Carrasco, L., Barbacid, M., Vázquez, D. 1973. The trichodermin group of antibiotics, inhibitors of peptide bond formation by eukaryotic polysomes. Biochimica et Biophysica Acta312:368–376.

    PubMed  Google Scholar 

  2. Carrasco, L., Jiménez, A., Vázquez, D. 1976. Specific inhibition of translocation by tubulosine in eukaryotic polysomes. European Journal of Biochemistry64:1–6.

    PubMed  Google Scholar 

  3. Carrasco, L., Vázquez, D. 1973. Differences in eukaryotic ribosomes detected by the selective action of an antibiotic. Biochimica et Biophysica Acta319:209–215.

    PubMed  Google Scholar 

  4. Carrasco, L., Vázquez, D. 1973. Ribosomal sites involved in the binding to aminoacyl-tRNA and EF-2: mode of action of fusidic acid. FEBS Letters32:152–156.

    PubMed  Google Scholar 

  5. Holzapfel, C. W. 1968. The isolation and structure of cyclopiazonic acid, a toxic metabolite ofPenicillium cyclopium Westling. Tetrahedron24:2101–2119.

    PubMed  Google Scholar 

  6. Jiménez, A., Carrasco, L., Vázquez, D. 1977. Enzymic and nonenzymic translocation by yeast polysomes: sites of action of a number of inhibitors. Biochemistry16:4727–4730.

    PubMed  Google Scholar 

  7. Jiménez, A., Sánchez, L., Vázquez, D. 1975. Simultaneous ribosomal resistance to trichodermin and anisomycin inSaccharomyces cerevisiae. Biochimica et Biophysica Acta383:427–434.

    PubMed  Google Scholar 

  8. Modolell, J., Vázquez, D. 1975. Polypeptide chain elongation and termination, pp. 137–178. In: Arnstein, H. R., (ed.), MTP international review of science, Biochemistry, Vol 7: Synthesis of amino acids and proteins, Oxford: Medical and Technical Publishing Co.

    Google Scholar 

  9. Neth, R., Monro, R. E., Heller C., Battaner, E., Vázquez, D. 1970. Catalysis of peptidyl transfer by human ribosomes and effects of some antibiotics. FEBS Letters6:198–202.

    PubMed  Google Scholar 

  10. Purchase, I. F. H. 1974. Mycotoxins. Amsterdam: Elsevier Scientific.

    Google Scholar 

  11. Zalacaín, M., Zaera, E., Vázquez, D., Jiménez, A. 1982. The mode of action of the antitumor drug bouvardin, an inhibitor of protein synthesis in eukaryotic cells. FEBS Letters148:95–97.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Center of Molecular Biology, Institute of Biochemistry of Macromolecules and Department of Microbiology, C.S.I.C. and U.A.M., Canto Blanco, Madrid-34, Spain

    E. Zaera, F. Santamaría, D. Vázquez & A. Jiménez

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  1. E. Zaera
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  2. F. Santamaría
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  3. D. Vázquez
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  4. A. Jiménez
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Zaera, E., Santamaría, F., Vázquez, D. et al. Inhibition of translation by the fungal toxin cyclopiazonic acid. Current Microbiology 9, 259–261 (1983). https://doi.org/10.1007/BF01567197

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  • DOI: https://doi.org/10.1007/BF01567197

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