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Cytochemical localization of acid phosphatases in the dimorphic fungusSporothrix schenckii

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Abstract

A modified Gomori procedure at the electron microscopic level revealed a multiplicity of acid phosphatase activity sites in both yeast-like and mycelial phase cells. Vacuoles and the periplasmic space contained electron opaque deposits (lead phosphate) that were absent in control incubations either lacking the substrate (β-glycerophosphate) or fortified with an inhibitor (sodium fluoride). The outermost region of the cell envelope was also active and, in contradistinction to previous examples with other yeasts, deposition of lead phosphate in this locale occurred even when the rate of orthophosphate generation was drastically reduced by lowering the substrate concentration. When mechanically disrupted yeast-like cells were washed and then subjected to the cytochemical procedure, pieces of broken cell envelope gave a positive reaction. The reaction product was invariably restricted to one side of cell wall cross sections. A specific and novel association of acid phosphatase with a microfibrillar zone was indicated.

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Literature Cited

  1. Antibus, R. K., Croxdale, J. G., Miller, O. K., Linkins, A. E. 1981. Ectomycorrhizic fungi ofSalix rotundifolia. 3. Resynthesized mycorrhizal complexes and their surface phosphatase activities. Canadian Journal of Botany59:2458–2465.

    Google Scholar 

  2. Arnold, W. N. 1974. Expression of cryptic β-fructofuranosidase inSaccharomyces rouxii. Journal of Bacteriology120:886–894.

    PubMed  Google Scholar 

  3. Arnold, W. N. 1981. Enzymes, pp. 1–46. In: Arnold, W. N. (ed.), Yeast cell envelopes: Biochemistry, biophysics, and ultrastructure, vol. II. Boca Raton, Florida: CRC Press.

    Google Scholar 

  4. Arnold, W. N., Garrison, R. G. 1979. An Fe+++-activated acid phosphatase inSaccharomyces rouxii. Journal of Biological Chemistry254:4919–4924.

    PubMed  Google Scholar 

  5. Arnold, W. N., Garrison, R. G. 1981. Kinetic limitations on the trapping of nascent phosphate for cytochemical localization of yeast acid phosphatase. Current Microbiology5:57–60.

    Google Scholar 

  6. Bartlett, E. M., Lewis, D. H. 1973. Surface phosphatase activity of mycorrhizal roots of beech. Soil Biology and Biochemistry5:249–257.

    Google Scholar 

  7. Benchimole, M., Souza, W., Travassos, L. R. 1979. Distribution of anionic groups at the cell surface of differentSporothrix schenckii cell types. Infection and Immunity24:912–919.

    PubMed  Google Scholar 

  8. Garrison, R. G. 1981. Vegetative ultrastructure. pp. 139–160. In: Arnold, W. N. (ed.), Yeast cell envelopes: biochemistry, biophysics, and ultrastructure, vol. II. Boca Raton, Florida: CRC Press.

    Google Scholar 

  9. Garrison, R. G. 1983. Ultrastructural cytology of pathogenic fungi, pp. 229–321. In: Howard, D. H. (ed.), Fungi pathogenic for human and animals: part A. New York: Marcel Dekker.

    Google Scholar 

  10. Garrison, R. G., Boyd, K. S., Mariat, F. 1975. Ultrastructural studies of the mycelium-to-yeast transformation ofSporothrix schenckii. Journal of Bacteriology124:959–968.

    PubMed  Google Scholar 

  11. Garrison, R. G., Mariat, F., Fromentin, H., Mirikitani, F. K. 1982. Electron microscopic analysis of yeastlike cell formation from the conidia ofSporothrix schenckii. Annales de Microbiologie (Inst. Pasteur)133B:189–204.

    Google Scholar 

  12. Lane, J. W., Garrison, R. G., Field, M. F. 1969. Ultrastructural studies on the yeast-like and mycelial phases ofSporotrichum schenckii. Journal of Bacteriology100:1010–1019.

    PubMed  Google Scholar 

  13. Travassos, L. R., Lloyd, K. O. 1980.Sporothrix schenckii and related species ofCeratocystis. Microbiological Reviews44:683–721.

    PubMed  Google Scholar 

  14. Tronchin, G., Poulain, D., Biguet, J. 1980. Localisation ultrastructurale de l'activité phosphatasique acide chezCandida albicans. Biologie Cellulaire38:147–152.

    Google Scholar 

  15. Wakeo, N., Sakurai, Y., Shiota, H., Tanimura, I. 1975. Cytochemical localization of acid and alkaline phosphatases inAspergillus oryzae, Journal of General and Applied Microbiology21:233–249.

    Google Scholar 

  16. Williamson, B., Alexander, I. J. 1975. Acid phosphatase localized in the sheath of beech mycorrhiza. Soil Biology and Biochemistry7:195–198.

    Google Scholar 

  17. Yamamoto, S., Minoda, Y., Yamada, Y. 1969. Localization of acid phosphatase activity in mycelia ofAspergillus terreus at electron-microscope level. Agricultural Biological Chemistry33:1351–1356.

    Google Scholar 

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Authors and Affiliations

  1. Research Service, Veterans Administration Medical Center, 64128, Kansas City, Missouri

    Robert G. Garrison

  2. Department of Microbiology and Department of Biochemistry, University of Kansas Medical Center, 66103, Kansas City, Kansas, USA

    Wilfred N. Arnold

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  1. Robert G. Garrison
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  2. Wilfred N. Arnold
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Garrison, R.G., Arnold, W.N. Cytochemical localization of acid phosphatases in the dimorphic fungusSporothrix schenckii . Current Microbiology 9, 253–257 (1983). https://doi.org/10.1007/BF01567196

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  • DOI: https://doi.org/10.1007/BF01567196

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