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Further studies of the β-lactamases ofBacteroides species

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Abstract

The β-lactamases of individual strains ofBacteroides fragilis, B. thetaiotaomicron, andB. melaninogenicus were examined to characterize their enzymatic activity and the relation between the periplasmic and cytoplasmic forms of the enzymes. Km and Vmax values indicate that all strains examined were very similar in terms of enzymatic activity with the antibiotics tested. Electrophoretic analysis and treatment with phospholipase D suggest the presence of a cytoplasmic form of the enzyme that is modified upon entry into the periplasmic space.

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Authors and Affiliations

  1. Chemical Industry Institute of Toxicology, PO Box 12137, 27709, Research Triangle Park, North Carolina, USA

    J. Michael Sherrill

  2. Hynson, Westcott, and Dunning, Baltimore, Maryland, USA

    Laurence R. McCarthy

Authors
  1. J. Michael Sherrill
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  2. Laurence R. McCarthy
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Sherrill, J.M., McCarthy, L.R. Further studies of the β-lactamases ofBacteroides species. Current Microbiology 11, 231–233 (1984). https://doi.org/10.1007/BF01567166

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