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Stability and zymogenic nature of chitin synthase fromCandida albicans

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Abstract

Chitin synthase preparations from both yeast and mycelialCandida albicans were chiefly in a zymogenic form, activatable by trypsin treatment. This was especially marked for preparations that had been solubilized with digitonin treatment. Endogenous activation of chitin synthase zymogen was observed over many days in preparations stored in glycerol (33%, wt/v) at−12°C and over many hours in preparations stored at 30°C. Gel chromatography of enzyme preparations suggested that zymogen was preferentially retarded on the columm matrices in comparison with active enzyme.

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Authors and Affiliations

  1. Department of Microbiology, Marischal College, University of Aberdeen, AB9 1AS, Aberdeen, UK

    John C. Hardy & Graham W. Gooday

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  1. John C. Hardy
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  2. Graham W. Gooday
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Hardy, J.C., Gooday, G.W. Stability and zymogenic nature of chitin synthase fromCandida albicans . Current Microbiology 9, 51–54 (1983). https://doi.org/10.1007/BF01567133

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