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Nitrogen assimilation in facultative methylotrophic bacteria

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Abstract

A study was done of the pathways of nitrogen assimilation in the facultative methylotrophsPseudomonas MA andPseudomonas AM1, with ammonia or methylamine as nitrogen sources and with methylamine or succinate as carbon sources. When methylamine was the sole carbon and/or nitrogen source, both organisms possessed enzymes of the glutamine synthetase/glutamate synthase pathway, but when ammonia was the nitrogen sourcePseudomonas AM1 also synthesized glutamate dehydrogenase with a pH optimum of 9.0, andPseudomonas MA elaborated both glutamate dehydrogenase (pH optimum 7.5) and alanine dehydrogenase (pH optimum 9.0). Glutamate dehydrogenase and glutamate synthase from both organisms were solely NADPH-dependent; alanine dehydrogenase was NADH-dependent. No evidence was obtained for regulation of glutamine synthetase by adenylylation in either organism, nor did glutamine synthetase appear to regulate glutamate dehydrogenase synthesis.

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Literature Cited

  1. Aharonowitz, Y., Friedrich, C. G. 1980. Alanine dehydrogenase of the β-lactam producerStreptomyces clavuligerus. Archives of Microbiology125:137–142.

    PubMed  Google Scholar 

  2. Anthony, C. 1982. The Biochemistry of Methylotrophs, pp. 251–259. London: Academic Press.

    Google Scholar 

  3. Bellion, E., AliKhan, M. Y., Romano, M. 1980. Transport of methylamine byPseudomonas sp. MA. Journal of Bacteriology142:786–790.

    PubMed  Google Scholar 

  4. Bellion, E., Bolbot, J. A., Lash, T. D. 1981. Generation of glyoxylate in methylotrophic bacteria. Current Microbiology6:367–372.

    Google Scholar 

  5. Bellion, E., Hersh, L. B. 1972. Methylamine metabolism in aPseudomonas species. Archives of Biochemistry and Biophysics153:368–374.

    PubMed  Google Scholar 

  6. Bellion, E., Kim, Y. S. 1978. Catabolite repression of isocitrate lyase in methylamine-grownPseudomonas MA. Effect of carbon and nitrogen sources. Biochimica et Biophysica Acta541:425–434.

    PubMed  Google Scholar 

  7. Bellion, E., Wayland, L. 1982. Methylamine uptake inPseudomonas sp. strain MA: Utilization of methylamine as the sole nitrogen source. Journal of Bacteriology149:395–398.

    PubMed  Google Scholar 

  8. Bender, R. A., Janssen, K. A., Resnick, A. D., Blumenberg, M., Foor, F., Magasanik, B. 1977. Biochemical parameters of glutamine synthetase fromKlebsiella aerogenes. Journal of Bacteriology129:1001–1009.

    PubMed  Google Scholar 

  9. Bolbot, J. A., Anthony, C. 1980. The metabolism of pyruvate by the facultative methylotrophPseudomonas AM1. Journal of General Microbiology120:233–244.

    Google Scholar 

  10. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Analytical Biochemistry72:248–254.

    PubMed  Google Scholar 

  11. Brenchley, J. E., Baker, C. A., Patil, L. G. 1975. Regulation of the ammonia assimilatory enzymes inSalmonella typhimurium. Journal of Bacteriology124:182–189.

    PubMed  Google Scholar 

  12. Brown, C. M., MacDonald-Brown, D. S., Stanley, S. O. 1973. The mechanisms of nitrogen assimilation in Pseudomonads. Antonie van Leeuwenhoek39:89–98.

    PubMed  Google Scholar 

  13. Dalton, H. 1979. Utilization of inorganic nitrogen by microbial cells. J. R. Quayle (ed.) In: International Review of Biochemistry, Vol.21, pp. 227–266. Baltimore: University Park Press.

    Google Scholar 

  14. Deuel, T. F., Stadtman, E. R. 1970. Some kinetic properties ofBacillus subtilis glutamine synthetase. Journal of Biological Chemistry245:5206–5213.

    PubMed  Google Scholar 

  15. Eady, R. R., Large, P. J. 1968. Purification and properties of an amine dehydrogenase fromPseudomonas AM1 and its role in growth on methylamine. Biochemical Journal168:245–255.

    Google Scholar 

  16. Hersh, L. B., Peterson, J. A., Thompson, A. A. 1971. An N-methylglutamate dehydrogenase fromPseudomonas MA. Archives of Biochemistry and Biophysics150:219–226.

    Google Scholar 

  17. Janssen, D. B., Hubb, J. M., Lennen, P. J. M., Van der Drift, C. 1980. The enzymes of ammonia assimilation inPseudomonas aeroginosa. Archives of Microbiology124:197–203.

    PubMed  Google Scholar 

  18. Lin, M. C. M., Wagner, C. 1975. Purification and characterization of N-methylalanine dehydrogenase. Journal of Biological Chemistry250:3746–3751.

    PubMed  Google Scholar 

  19. Loginova, N. V., Govorukhina, N. I., Trotsenko, Y. A. 1982. Enzymes of the assimilation of ammonium in bacteria with various pathways of C1-metabolism. Mikrobiologiya51:38–42 (English translation).

    Google Scholar 

  20. Magasanik, B., Prival, M. J., Brenchley, J. A., Tyler, B. M., Deleo, A. B., Streicher, S. L., Bender, R. A., Paris, C. G. 1974. Glutamine synthetase as a regulator of enzyme synthesis, pp. 119–138. In Horecker. B. Stadtman, E. (eds.), Current Topics in Cellular Regulation,8. New York: Academic Press Inc.

    Google Scholar 

  21. McCowen, S. M., Phibbs, P. V. 1974. Regulation of alanine dehydrogenase inBacillus lichenformis. Journal of Bacteriology118:590–597.

    PubMed  Google Scholar 

  22. Meers, J. L., Tempest, D. W., Brown, C. M. 1970. Glutamine (amide): 2-oxoglutarate aminotransferase oxidoreductase (NADP), an enzyme involved in the synthesis of glutamate by some bacteria. Journal of General Microbiology64:187–194.

    PubMed  Google Scholar 

  23. Meyer, J. M., Stadtman, E. R. 1981. Glutamine synthetase of pseudomonads. Some biochemical and physicochemical properties. Journal of Bacteriology146:705–712.

    PubMed  Google Scholar 

  24. Murrell, J. C., Dalton, H. 1983. Purification and properties of glutamine synthetase fromMethylococuus capsulatus (Bath). Journal of General Microbiology129:1187–1196.

    Google Scholar 

  25. Murrell, J. C., Dalton, H. 1983. Ammonia assimilation inMethylococcus capsulatus (Bath) and other obligate methanotrophs. Journal of General Microbiology129:1197–1206.

    Google Scholar 

  26. Nyberg, K., Clarke, P. H. 1978. Glutamine synthetase activities of cultures ofPseudomonas aeruginosa grown in minimal media with histidine, nitrate, or ammonium sulfate as the nitrogen source. Journal of General Microbiology107:193–197.

    Google Scholar 

  27. Orr, J., Haselkorn, R. 1982. The regulation of glutamine synthetase activity and synthesis in free-living and symbioticAnabeana spp. Journal of Bacteriology152:626–635.

    PubMed  Google Scholar 

  28. Peel, D., Quayle, J. R. 1961. Microbial growth on C1 compounds. 1. Isolation and characterization ofPseudomonas AM1. Biochemical Journal81:465–469.

    PubMed  Google Scholar 

  29. Pollock, R. J., Hersh, L. B. 1971. N-methylglutamate synthetase. Purification and properties of the enzyme. Journal of Biological Chemistry246:4737–4743.

    PubMed  Google Scholar 

  30. Rowell, P., Endicott, S., Stewart, W. D. P. 1977. Glutamine synthetase and nitrogenase activity in the blue-green algaAnabaena cylindrica. New Phytology79:41–54.

    Google Scholar 

  31. Rowell, P., Stewart, W. D. P. 1976. Alanine dehydrogenase of the N2-fixing blue-green algaAnabaena cylindrica. Archives of Microbiology107:115–124.

    Google Scholar 

  32. Salem, A. R., Hacking, A. J., Quayle, J. R. 1973. Cleavage of malyl CoA into acetyl CoA and glyoxylate byPseudomonas AM1 and other C1-unit utilizing bacteria. Biochemical Journal136:89–96.

    PubMed  Google Scholar 

  33. Shapiro, B. M., Stadtman, E. R. 1970. Glutamine synthetase (Escherichia coli). In: Colowick, S. P., Kaplan, N. O. (eds.), Methods in Enzymology,17A, pp. 910–922. London and New York: Academic Press, Inc.

    Google Scholar 

  34. Senior, P. J. 1975. Regulation of nitrogen metabolism inEscherichia coli andKlebsiella aerogenes: Studies with the continuous culture technique. Journal of Bacteriology123:407–418.

    PubMed  Google Scholar 

  35. Shaw, W. V., Tsai, L., Stadtman, E. R. 1966. The enzymatic synthesis of N-methylglutamic acid. Journal of Biological Chemistry241:935–945.

    PubMed  Google Scholar 

  36. Shishkina, V. N., Trotsenko, Y. A. 1979. Pathways of ammonia assimilation in obligate methane utilizers. FEMS Microbiology Letters5:187–191.

    Google Scholar 

  37. Stacey, G., Van Baalen, C., Tabita, F. R. 1979. Nitrogen and ammonia assimilation in the cyanobacteria: Regulation of glutamine synthetase. Archives of Biochemistry and Biophysics194:457–467.

    PubMed  Google Scholar 

  38. Stadtman, E. R., Ginsburg, A., Ciardi, E., Yeh, S., Hennig, B., Shapiro, B. M. 1970. Multiple molecular forms of glutamine synthetase produced by enzyme-catalyzed adenylylation and deadenylylation reactions. Advances in Enzyme Regulation8:99–118.

    PubMed  Google Scholar 

  39. Streicher, S. L., DeLeo, A. B., Magasanik, B. 1976. Regulation of enzyme formation inKlebsiella aerogenes by episomal glutamine synthetase ofEscherichia coli. Journal of Bacteriology127:184–192.

    PubMed  Google Scholar 

  40. Tuli, R., Thomas, J. 1981.In vivo regulation of glutamine synthetase by ammonium in the cyanobacteriumAnabaena L-31. Archives of Biochemistry and Biophysics206:181–189.

    PubMed  Google Scholar 

  41. Tyler, B. 1978. Regulation of the assimilation of nitrogen compounds. Annual Review of Biochemistry47:1127–1162.

    PubMed  Google Scholar 

  42. Wedler, F. C., Carfi, J., Ashour, A. E. 1976. Glutamine synthetase ofBacillus stearothermophilus. Regulation, site interactions and functional information. Biochemistry15:1749–1755.

    PubMed  Google Scholar 

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  1. Department of Chemistry, University of Texas at Arlington, 76019, Arlington, Texas

    Edward Bellion & John A. Bolbot

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  1. Edward Bellion
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  2. John A. Bolbot
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Bellion, E., Bolbot, J.A. Nitrogen assimilation in facultative methylotrophic bacteria. Current Microbiology 9, 37–44 (1983). https://doi.org/10.1007/BF01567131

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