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Use of the Mud(Ap,lac) bacteriophage to study the regulation of L-proline biosynthetic genes inEscherichia coli K-12

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Abstract

One operon fusion to the promoter of either theproA orproB genes of the proline biosynthetic pathway was obtained by the use of the Mud(Ap,lac) bacteriophage. This operon fusion was further stabilized by transformation with the plasmid pGW600 containing the wild type Mu repressor gene. The level of β-galactosidase in this strain was not affected by the presence of high concentrations of NaCl in the growth medium. Mutations affecting the regulation of thispro-lac genetic fusion were generated by the insertion of Tn5; β-galactosidase levels in these mutants were higher than in the parental strain when proline was present at a high level. In some of these mutants we observed either repression or maintenance of β-galactosidase levels whenpro-lac (F′proAB +) merodiploids were constructed.

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Authors and Affiliations

  1. Departmento de Higiene y Microbillogia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid-3, Spain

    Pablo E. Hernandez, Juan A. Ordoñez & B. Sanz Perez

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  1. Pablo E. Hernandez
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  2. Juan A. Ordoñez
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  3. B. Sanz Perez
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Hernandez, P.E., Ordoñez, J.A. & Sanz Perez, B. Use of the Mud(Ap,lac) bacteriophage to study the regulation of L-proline biosynthetic genes inEscherichia coli K-12. Current Microbiology 9, 31–35 (1983). https://doi.org/10.1007/BF01567130

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