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Sensitivity of coliphage T1 to nickel in fresh and salt waters

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Abstract

Coliphage T1 was more sensitive than its host bacterium,Escherichia coli B, to nickel (Ni). A 5-h exposure to 100 ppm Ni in nutrient broth did not adversely affect T1, whereas 10 and 20 ppm Ni extended the lag phase of growth ofE. coli B, and no growth occurred with 40 or more ppm Ni. 5 ppm Ni enhanced the survival (after 4 wk) of T1 in sea or simulated estuarine water but was toxic (i.e., reduced viral infectivity) in lake water; 50 ppm Ni was not toxic to T1 in sea water, was moderately toxic in estuarine water, but was highly toxic in lake water; and 100 ppm Ni was toxic in all systems, with the sequence of loss in viral infectivity being lake > estuarine > sea water. 100 ppm Ni was not toxic to T1 in nutrient broth, even after 3 wk of exposure, probably because of the protective effect of the organic compounds in the broth.

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Literature Cited

  1. Akin, E. W., Hill, F., Jr., Clarke, N. A. 1975. Mortality of enteric viruses in marine and other wastes, pp. 1–9. In: Proceedings of the International Symposium on Discharge of Sewage from Sea Outfalls. Elmsford, N.Y.: Pergamon Press.

    Google Scholar 

  2. Babich, H., Stotzky, G. 1978. Toxicity of zinc to fungi, bacteria, and coliphages: influence of chloride ions. Applied and Environmental Microbiology36:904–913.

    Google Scholar 

  3. Babich, H., Stotzky, G. 1979. Differential toxicities of mercury to bacteria and bacteriophages in sea and in lake water. Canadian Journal of Microbiology25:1252–1257.

    PubMed  Google Scholar 

  4. Babich, H., Stotzky, G. 1980. Environmental factors that influence the toxicity of heavy metals and gaseous pollutants to microorganisms. Critical Reviews in Microbiology8:99–145.

    PubMed  Google Scholar 

  5. Babich, H., Stotzky, G. 1980. Reductions in inactivation rates of bacteriophages by clay minerals in lake water. Water Research14:185–187.

    Google Scholar 

  6. Babich, H., Stotzky, G., 1981. Components of water hardness that reduce the toxicity of nickel to fungi. Microbios Letters18:17–24.

    Google Scholar 

  7. Babich, H., Stotzky, G. 1982. Nickel toxicity to microbes: effect of pH and implications for acid rain. Environmental Research29:335–350.

    PubMed  Google Scholar 

  8. Babich, H., Stotzky, G. 1982. Nickel toxicity to estuarine/marine fungi and its amelioration by magnesium in sea water. Water, Air, and Soil Pollution (in press).

  9. Babich, H., Stotzky, G. 1983. Temperature, pH, salinity, hardness and particulates mediate nickel toxicity to eubacteria, an actinomycete, and yeasts in lake, simulated estuarine, and seawater. Aquatic Toxicology (in press).

  10. Babich, H., Stotzky, G. 1983. Further studies on environmental factors that modify the toxicity of nickel to microbes. Regulatory Toxicology and Pharmacology (in press).

  11. Berry, S. A., Norton, B. G. 1977. Survival of bacteriophages in seawater. Water Research8:323–327.

    Google Scholar 

  12. Bitton, G., Mitchell, R. 1974. Effect of colloids on the survival of bacteriophages in seawater. Water Research8:227–229.

    Google Scholar 

  13. Blundell, M. R., Wild, D. G. 1969. Inhibition of bacterial growth by metal salts. Biochemical Journal115:207–211.

    PubMed  Google Scholar 

  14. Corbett, T. H., Heidelberger, C., Dove, W. F. 1970. Determination of the mutagenic activity to bacteriophage T4 of carcinogenic and noncarcinogenic compounds. Molecular Pharmacology6:667–679.

    PubMed  Google Scholar 

  15. Fishesjo, G. 1971. The effect of two mercury compounds on lysogenicE. coli K 39 (λ). Hereditas69:135–138.

    PubMed  Google Scholar 

  16. Gonye, E. R., Jr., Jones, G. E. 1973. An ecological survey of open ocean and estuarine microbial populations. II. The oligodynamic effect of nickel on marine bacteria, pp. 243–257. In: Stevenson, H., Colwell, R. R. (eds.), Estuarine microbial ecology. Columbia, South Carolina: University of South Carolina Press.

    Google Scholar 

  17. Guha, C., Mookerjee, A. 1979. Effect of nickel on macromolecular synthesis inEscherichia coli K-12. Nucleus22:45–47.

    Google Scholar 

  18. Hermann, J. E., Kostenbader, K. D., Jr., Clive, D. O. 1974. Persistence of enteroviruses in lake water. Applied Microbiology28:895–896.

    PubMed  Google Scholar 

  19. Kozloff, L. M. 1978. Properties of T4D bacteriophage grown in synthetic media containing Zn2+, Co2+, or Ni2+. Journal of Biological Chemistry253:1059–1064.

    PubMed  Google Scholar 

  20. Kozloff, L. M., Lute, M., Henderson, K. 1957. Viral invasion. Rupture of thiol ester bonds in the bacteriophage tail. Journal of Biological Chemistry228:511–528.

    PubMed  Google Scholar 

  21. Lo, S., Gilbert, J., Hetrick, F. 1976. Stability of enteroviruses in estuarine and marine waters. Applied and Environmental Microbiology32:245–249.

    PubMed  Google Scholar 

  22. Mitchell, R., Jaanasch, H. W. 1969. Processes controlling virus inactivation in seawater. Environmental Science and Technology3:941–943.

    Google Scholar 

  23. Orgel, A., Orgel, L. E. 1965. Induction of mutations in bacteriophage T4 with divalent manganese. Journal of Molecular Biology14:453–457.

    PubMed  Google Scholar 

  24. Puck, T. T., Garen, A., Cline, J. 1951. The mechanism of virus attachment to host cells. I. The role of ions in the primary reaction. Journal of Experimental Medicine93:65–88.

    PubMed  Google Scholar 

  25. Schiffenbauer, M., Stotzky, G. 1982. Absorption of coliphages T1 and T7 to clay minerals. Applied and Environmental Microbiology43:590–596.

    PubMed  Google Scholar 

  26. Stent, G. S. 1963. Molecular biology of bacterial viruses. San Francisco, California: W. H. Freeman and Co.

    Google Scholar 

  27. Tzagoloff, H., Pratt, D. 1964. The initial steps in infection with coliphage M13. Virology24:372–380.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Microbial Ecology, Department of Biology, New York University, Washington Square, 100003, New York, New York

    H. Babich & G. Stotzky

  2. Department of Biology, Pace University, 10038, New York, New York

    M. Schiffenbauer

Authors
  1. H. Babich
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  2. M. Schiffenbauer
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  3. G. Stotzky
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Babich, H., Schiffenbauer, M. & Stotzky, G. Sensitivity of coliphage T1 to nickel in fresh and salt waters. Current Microbiology 8, 101–105 (1983). https://doi.org/10.1007/BF01566966

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