Abstract
Production of a blue pigment, indigoidine, is a variable trait among wild-type strains ofErwinia chrysanthemi; it is also influenced by the composition of the growth medium. Starting with a nonpigmented wild-type strain (ICPB EC183) ofE. chrysanthemi, we obtained by nitrosoguanidine mutagenesis a pigmented (idg +) mutant strain (AC6055), which simultaneously was Arg−. Linkage betweenarg andidg was established in two-factor transductional and conjugational crosses. Coinheritance ofidg withhis +,ilv +,leu +,ser +,thr +, orura + transductants was not observed. Spontaneous Arg+ revertants of AC6055 were invariably Idg+. The pigments produced by AC6055, Idg+ recombinants, and wild-type Idg+ strains were identical, judged by absorption spectra (λmax = 615 nm) of the dimethylsulfoxide extracts of whole cells. We concluded that nitrosoguanidine caused comutation in thearg andidg loci that are linked on theE. chrysanthemi chromosome.
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Chatterjee, A.K., Brown, M.A. Chromosomal location of a gene (idg) that specifies production of the blue pigment indigoidine inErwinia chrysanthemi . Current Microbiology 6, 269–273 (1981). https://doi.org/10.1007/BF01566875
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DOI: https://doi.org/10.1007/BF01566875