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Lack of genetic relatedness among animal and plant acholeplasmas by nucleic acid hybridization

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Abstract

The present study compared the genetic characteristics of three previously unclassified acholeplasmas of plant origin with those of the eight established species ofAcholeplasma. A radiolabeled DNA probe was derived from the lemon (L1) strain acholeplasma by using the nick translation method and hybridized to the excess unlabeled DNA isolated from the eight established species ofAcholeplasma and three new isolates from plants. Nucleic acid hydridization and serological tests confirmed that the L1 acholeplasma was distinct from all eight established species ofAcholeplasma but closely related to strains isolated from the flowers of grapefruit trees (GF1) and powder puff plants (PP2). The results suggest that these new acholeplasmas isolated exclusively from plants may represent a new species ofAcholeplasma.

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Authors and Affiliations

  1. Mycoplasma Branch, Bureau of Biologics, Food and Drug Administration, 20205, Bethesda, Maryland, USA

    Edward B. Stephens & Michael F. Barile

  2. Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, 20214, Bethesda, Maryland, USA

    Gurmit S. Aulakh

  3. University of Florida Agricultural Research Center, 3205 Southwest College Avenue, 33314, Fort Lauderdale, Florida, USA

    Randolph E. McCoy

  4. Mycoplasma Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infections Diseases, 20205, Bethesda, Maryland, USA

    David L. Rose & Joseph G. Tully

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  1. Edward B. Stephens
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  2. Gurmit S. Aulakh
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  3. Randolph E. McCoy
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  4. David L. Rose
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  5. Joseph G. Tully
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  6. Michael F. Barile
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Stephens, E.B., Aulakh, G.S., McCoy, R.E. et al. Lack of genetic relatedness among animal and plant acholeplasmas by nucleic acid hybridization. Current Microbiology 5, 367–370 (1981). https://doi.org/10.1007/BF01566751

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