Skip to main content
SpringerLink
Log in

Influence of nicotinic acid on insulin release in vitro

  • Lavori Originali
  • Published:
Acta diabetologia latina Aims and scope Submit manuscript

Riassunto

L'acido nicotinico stimola la liberazione di insulina da parte di isole isolate di topo, in rapporto alla concentrazione del farmaco, ma indipendentemente dell'apporto di glucosio. La nicotinamide ed i nucleotidi piridinici sono privi di effetto sulla liberazione dell'ormone. La 6-amino-nicotinamide inibisce la liberazione insulinica stimolata dal glucosio. Questi risultati vengono discussi in relazione all'importanza delloshunt dei pentoso-fosfati per la regolazione della secrezione insulinica.

Résumé

L'acide de nicotine stimule la libération d'insuline de la part d'îlots isolés de rat, par rapport à la concentration du médicament, mais indépendamment de l'apport de glucose. La nicotinamide et les nucléotides pyridiniques n'ont aucun effet sur la libération de l'hormone. La 6-amino-nicotinamide inhibe la libération d'insuline stimulée par le glucose. Ces résultats sont examinés par rapport à l'importance dushunt des pentose-phosphates pour la régularisation de la sécrétion insulinique.

Resumen

El ácido nicotínico estimula la liberación de insulina por parte de islas aisladas de ratón, en relación con la concentración del producto, pero independientemente de la aportación de glucosa. La nicotinamida y los nucleótidos piridínicos carecen de efecto sobre la liberacción del hormón. La 6-amino-nicotinamida inhibe la liberación de insulina estimulada por glucosa. Estos resultados se discuten en relación con la importancia delshunt de los pentosa-fosfatos para la regulación de la secreción insulínica.

Zusammenfassung

Nicotinsäure stimuliert glucoseunabhängig, aber dosisabhängig, die Insulinsekretion isolierter Mäuseinselnin vitro. Nicotinamid, wie auch die Pyridinnucleotide haben keine Wirkung, auf die Hormonabgabe, während 6-Aminonicotinamid die glucose-stimulierte Insulinsekretion hemmt. Die erhobenen Befunde werden im Zusammenhang mit der Bedeutung des Pentosephosphatshunts für die Regulation der Insulinsekretion diskutiert.

Summary

Nicotinic acid stimulates insulin output of isolated mice islets in correlation with the drug concentration but regardless of glucose supply. Nicotinamide and also the pyridine nucleotides were ineffective on the insulin output. 6-amino-nicotinamide inhibits the glucose-stimulated insulin release. The results are discussed in connexion with the significance of the pentose phosphate shunt for the regulation of insulin secretion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bekierkunst A., Windman A. J.: Nicotinic Acid Induced NAD Synthesis in Polymorphonuclear Leucocytes - Life Sci.7 539, 1968.

    Article  CAS  Google Scholar 

  2. Bibergeil H., Michaelis D., Zander H., Wulfert P., Jutzi E.: Inhibition of Lipolysis with 5-Methylpyrazole-3-Carboxylic Acid and its Influence on Insulin Efficiency in Carbohydrate Metabolism in Diabetics - In: Reports on Oral Diabetes Therapy Especially with HB 419. Excerpta Medica Foundation, Amsterdam, 1971; p. 41.

    Google Scholar 

  3. Björntorp P.: The Effect of Nicotinic Acid on Adipose Tissue Metabolism in Vitro -Metabolism14 836, 1965.

    Article  PubMed  Google Scholar 

  4. Carlson L. A.: Studies on the Effect of Nicotinic Acid on Catecholamine Stimulated Lipolysis in Adipose Tissue in Vitro - Acta med. scand.173 719, 1963.

    Article  CAS  PubMed  Google Scholar 

  5. Carlson L. A., Nye E. R.: Acute Effects of Nicotinic Acid in the Rat -Acta med. scand.179 453, 1966.

    Article  CAS  PubMed  Google Scholar 

  6. Carlson L. A., Öro L., Östman J.: Effect of a Single Dose of Nicotinic Acid on Plasma Lipids in Patients with Hyperlipoproteinemia - Acta med. scand.183 457, 1968.

    Article  CAS  PubMed  Google Scholar 

  7. Dalton C., van Trabert T. C., Dwyer J. X.: Relationship of Nicotinamide and Nicotinic Acid to Hypolipidemia - Biochem. Pharmacol.19 2609, 1970.

    Article  CAS  PubMed  Google Scholar 

  8. Ginoulhiac E., Tenconi L. T., Chiancone F. M.: 2-Pyridin Acetic Acid and Nicotinic Acid: Blood Levels, Urinary Elimination and Excretion of Nicotinic Acid Derivatives in Man -Nature (Lond.)193 948, 1962.

    Article  CAS  Google Scholar 

  9. Greengard P., Quinn G. P., Reid M. B.: Pituitary Influence on Pyridine Nucleotide Metabolism in Rat Liver - J. biol. Chem.239 1887, 1966.

    Google Scholar 

  10. Gross R. C., Carlson L. A.: Metabolic Effects of Nicotinic Acid in Acute Insulin Deficiency in the Rat - Diabetes17 353, 1968.

    Article  CAS  PubMed  Google Scholar 

  11. Gumaa K. A., McLean P.: Effect of Oxamate, Pyruvate, Nicotinamide and Streptozotocin on the Pentose Phosphate Pathway Intermediates in Ascites Tumour Cells - Biochem. biophys. Res. Commun.35 86, 1969.

    Article  CAS  PubMed  Google Scholar 

  12. Hahn H.-J., Lippmann H. G., Knospe S., Michael R.: Verhalten der Langerhans'schen Inseln des Pankreas in vitro. III. - Acta biol. med. germ.25 573, 1970.

    CAS  PubMed  Google Scholar 

  13. Hahn H.-J., Lippmann H. G., Schultz D.: Verhalten der Langerhans'schen Inseln des Pankreas in vitro. I. - Acta biol. med. germ.25 421, 1970.

    CAS  PubMed  Google Scholar 

  14. Hales C. N., Randle P. J.: Immunoassay of Insulin with Insulin Antibody Precipitate -Biochem. J.88 137, 1963.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Haller H., Strauzenberg S. E.: Zur Frage der Wirksamkeit der Nikotinsäure auf den Kohlenhydratstoffwechsel - Z. ges. inn. Med.12 954, 1957.

    CAS  PubMed  Google Scholar 

  16. Herken H., Lange K.: Blocking of Pentose Phosphate Pathway in the Brain of Rats - Naunyn-Schmiedeberg's Arch. Pharmak. exp. Path.263 496, 1969.

    Article  CAS  Google Scholar 

  17. Ho R. J., Jeanrenaud B.: Insulin-Like Action of Ouabain. I. Effect on Carbohydrate Metabolism- Biochim. biophys. Acta (Amst.)144 61, 1967.

    Article  CAS  Google Scholar 

  18. Lange K., Proft E. R.: Inhibition of the 6-Phosphogluconate Dehydrogenase in the Rat Kidney by 6-Aminonicotinamide - Naunyn-Schmiedeberg's Arch. Pharmak. exp. Path.267 177, 1970.

    Article  CAS  Google Scholar 

  19. Lee H. M., Ellis R. M., Sigal M. V.: Some Insulin-Like Effects of Nicotinic Acid Observed with Isolated Rat Epididymal Adipose Tissue - Biochim. biophys. Acta (Amst.)49 408, 1961.

    Article  CAS  Google Scholar 

  20. Lison H.: Untersuchungen zur Differenzierung der durch 6-Aminonicotinamid, einem Antimetaboliten des Nicotinamids, hervorgerufenen zentralnervösen Funktionsstörungen - Naunyn-Schmiedeberg's Arch. Pharmak. exp. Path.267 155, 1970.

    Article  CAS  Google Scholar 

  21. Lowry O. H., Rosenbrough N. J., Farr A. L., Randall P. J.: Protein Measurement with the Folin Reagent - J. biol. Chem.193 265, 1951.

    CAS  PubMed  Google Scholar 

  22. Luyckx A.: 1969. (Quoted byMiettinen T. A. et al.27).

  23. Malaisse W., Malaisse-Lagae F.: Effets du glucose sur la sécrétion pancréatique d'insuline in vitro dans différentes espèces de rongeurs - Ann. Endocr. (Paris)29 501, 1968.

    CAS  Google Scholar 

  24. Malaisse W., Malaisse-Lagae F., Mayhew D.: A Possible Role for the Adenylcyclase in Insulin Secretion - J. clin. Invest.46 1724, 1967.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Michaelis D.: Habilitationsschrift, Greifswald, 1971.

  26. Michaelis D., Hahn H.-J., Michael R., Knospe S., Schäfer H., Jutzi E., Wulfert P.: Effekte der intravenösen Nikotinsäureinfusion auf Substrat-Metabolit-, und Hormonkonzentrationen in Blut beim juvenil manifestierten Diabetes mellitus - Diabetologia6 550, 1970.

    Article  CAS  PubMed  Google Scholar 

  27. Miettinen T. A., Taskinen M. R., Pelkonen R., Nikkilä E. A.: Glucose Tolerance and Plasma Insulin in Man during Acute and Chronic Administration of Nicotinic Acid - Acta med. scand.186 247, 1969.

    Article  CAS  PubMed  Google Scholar 

  28. Montague W., Taylor K. W.: Pentitols and Insulin Release by Isolated Rat Islets of Langerhans- Biochem. J.109 333, 1968.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Paul P., Issekutz B. Jr.,Miller H.: Interrelationship of Free Fatty Acids and Glucose Metabolism in the Dog - Amer. J. Physiol.211 1313, 1966.

    CAS  PubMed  Google Scholar 

  30. Pereira J. N., Holland J. F.: The Effect of Nicotinamide Adenine Dinucleotide on Lipolysis in Adipose Tissue in Vitro - Experientia (Basel)22 658, 1966.

    Article  CAS  Google Scholar 

  31. Roth M. A., Ashmore J.: The Hypoglycemic Activity of Nicotinic Acid in Rats - Naunyn-Schmiedeberg's Arch. exp. Path. Pharmak.248 117, 1964.

    Article  Google Scholar 

  32. Schultz G., Senft G., Losert W., Schacht U.: Zum Mechanismus der 6-Aminonicotinsäureamid-Hyperglykämie- Naunyn-Schmiederberg's Arch. exp. Path. Pharmak.253 345, 1966.

    Article  CAS  Google Scholar 

  33. Watkins D., Cooperstein S. J., Dixit P. K., Lazarow A.: Insulin Secretion from Toadfish Islet Tissues Stimulated by Pyridine Nucleotides - Science162 283, 1968.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zentralinstitut für Diabetes «Gerhardt Katsch», 2201, Karlsburg/Greifswald, Deutsche Demokratische Republik

    Hans-Jürgen Hahn & Rainer Michael

Authors
  1. Hans-Jürgen Hahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rainer Michael
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hahn, HJ., Michael, R. Influence of nicotinic acid on insulin release in vitro. Acta diabet. lat 9, 87–96 (1972). https://doi.org/10.1007/BF01564541

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01564541

Key-words

Search

Navigation