Skip to main content
SpringerLink
Log in

T(Iat)- and B(Iab)-cell alloantigens determined by theH-2 linkedI region in mice

  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

The specificity of an antiserum directed againstI region associated (Ia) antigens is described. The serum was raised in (DBA/1×B10.D2)F1 mice against lymphocytes of AQR mice, differing from the responder for theI region only. The serum reacts with Ia antigens expressed on B cells (Iab) as well as with Ia antigens expressed on T cells (Iat). Absorption studies indicate that B cells possess at least two Ia antigens, and one of these is shared by T cells. However, this ‘shared antigen’ is not present on the surface of lymphocytes of thymectomized mice. Analysis of the strain distribution of Iab and Iat antigens revealed that the Iab antigens are present on lymphocytes of mice carrying theIA k subregion and that the Iat antigens are present on lymphocytes of mice carryingI region genes of theH-2 k haplotype located between theIA andIB subregions. This conclusion is based on the analysis of the antiserum's reactivity with T and B cells of the strains B10.A(2R), B10.A(4R) and B10.HTT: the serum reacts with B and T cells of B10.A(2R) but only with B cells of B10.A(4R) mice and only weakly with T cells of B10.HTT mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ALG:

antimouse lymphocyte globulin from rabbits

B cells:

bone marrow derived lymphocytes

B10:

C57BL/10Sn mice

D1D2F1 :

(DBA/1×B10.D2)F1 hybrid mice

GVHR:

graft-vs-host reaction

Ia:

I region associated antigen

Iab:

on B cells

Iat:

on T cells

MLR:

mixed lymphocyte reaction

T cells:

thymus-derived lymphocytes

Thy-1:

thymus antigen 1, formerly called theta

Tx-Lyc:

lymphocytes of thymectomized, ALG treated, lethally irradiated and anti-Thy-1 treated bone marrow reconstituted mice

2R:

B10.A(2R)/SgSn mice

4R:

B10.A(4R) mice

References

  • Alter, B. J., Schnedel, D. J., Bach, M. L., Bach, F. H., Klein, J., and Stimpfling, J. H.: Cell-mediated lympholysis. Importance of serologically definedH-2 regions.J. Exp. Med. 137: 1303–1309, 1973.

    PubMed  Google Scholar 

  • Amos, D. B., Bashir, H., Boyle, W., McQueen, M., and Tiilikainen, A.: A simple microcytotoxicity test.Transplantation 7: 220–222, 1969.

    PubMed  Google Scholar 

  • Bach, F. H., Widmer, M. B., Segall, M., Bach, M. L., and Klein, J.: Genetic and immunologic complexity of major histocompatibility regions.Science 176: 1024–1027, 1972a.

    PubMed  Google Scholar 

  • Bach, F. H., Widmer, M. B., Bach, M. L., and Klein, J.: Serologically defined and lymphocyte defined components of the major histocompatibility complex of the mouse.J. Exp. Med. 136: 1430–1444, 1972b.

    Google Scholar 

  • Benacerraf, B. and McDevitt, H. O.: The histocompatibility linked immune response genes.Science 175: 273–279, 1972.

    PubMed  Google Scholar 

  • David, C. S., Shreffler, D. C., and Frelinger, J. A.: New lymphocyte antigen system (Lna) controlled by theIr region of the mouseH-2 complex.Proc. Nat. Acad. Sci USA 70: 2509–2514, 1973.

    Google Scholar 

  • Götze, D., Reisfeld, R. A., and Klein, J.: Serologic evidence for antigens controlled by the Ir region in mice.J. Exp. Med. 138: 1003–1008, 1973.

    Google Scholar 

  • Götze, D. and Reisfeld, R. A.: Soluble murine antigens. I. Immunogenicity of soluble H-2 antigens extracted with hypertonic salt solution.J. Immunol., 112: 1643–1651, 1974a.

    PubMed  Google Scholar 

  • Götze, D. and Reisfeld, R. A.: Soluble murine transplantation antigens. II. Immune response to H-2Kd antigens. Z.Immunitatsforsch., 148: 45–61, 1974b.

    Google Scholar 

  • Hämmerling, G. J., Deak, B. D., Mauve, G., Hammerling, V., and McDevitt, H. O.: B lymphocyte alloantigens controlled by the I region of the major histocompatibility complex in mice.Immunogenetics 1: 68–81, 1974.

    Google Scholar 

  • Hauptfeld, V., Klein, D., and Klein, J.: Serologic identification of anIr region product.Science 181: 167–169, 1973.

    PubMed  Google Scholar 

  • Julius, M. H., Simpson, E., and Herzenberg, L. A.: A rapid method for the isolation of functional thymus-derived murine lymphocytes.Eur. J. Immunol. 3: 645–649, 1973.

    PubMed  Google Scholar 

  • Klein, J.: The H-2 system. Past and Present.Transplant. Proc., 5: 11–21, 1973a.

    PubMed  Google Scholar 

  • Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. Springer, New York, N. Y., in press, 1974.

    Google Scholar 

  • Klein, J. and Park, J. M.: Graft versus host reaction across different regions of theH-2 complex of the mouse.J. Exp. Med. 137: 1213–1255, 1973.

    Google Scholar 

  • Klein, J., Hauptfeld, M., and Hauptfeld, V.: Evidence for a third,Ir-associated histocompatibility region in theH-2 complex of the mouse.Immunogenetics 1: 45–56, 1974a.

    Google Scholar 

  • Klein, J., Bach, F. H., Festenstein, H., McDevitt, H. O., Shreffler, D. C., Snell, G. D., and Stimpfling, J. H.: Genetic nomenclature for theH-2 complex of the mouse.Immunogenetics 1: 184–188, 1974b.

    Google Scholar 

  • Liebermann, R. and Humphrey, Jr. W.: Association of H-2 types with genetic control of immune responsiveness to IgG (j2a) allotypes in the mouse.J. Exp. Med. 136: 1222–1230, 1972.

    Google Scholar 

  • Liebermann, R., Paul, W. E., Humphrey, Jr., W., and Stimpfling, J. H.:H-2 linked immune response (Ir) genes.J. Exp. Med. 136: 1231–1240, 1972.

    PubMed  Google Scholar 

  • Livnat, S., Klein, J., and Bach, F. H.: Graft-versus-host reaction in strains of mice identical for H-2K and H-2D antigens.Nature New Biol 243: 42–44, 1973.

    Google Scholar 

  • McDevitt, H. O., Deak, B. D., Shreffler, D. C., Klein, J., Stimpfling, J. H., and Snell, G. D.: Genetic control of the immune response. Mapping of theIr-I locus.J. Exp. Med. 135: 1259–1278, 1972.

    Google Scholar 

  • Meo, T., Vives, J., Miggiano, V., and Shreffler, D. C.: A major role for theIr-region of the mouseH-2 complex in the mixed leukocyte reaction.Transplant. Proc. 5: 377–381, 1973a.

    PubMed  Google Scholar 

  • Meo, T., Vives, G., Rijnbeek, A. M., Miggiano, V. C., Nabholz, M., and Shreffler, D. C.: A bipartite interpretation and tentative mapping of H-2 associated MLR determinants in the mouse.Transplant. Proc. 5: 1339–1350, 1973b.

    PubMed  Google Scholar 

  • Meo, T., David, C. S., Nabholz, M., Miggiano, V., and Shreffler, D. C.: Crossover in the B10.HTT strain: implications concerning location of MLR determinants in theIr region.Transplant. Proc. 5: 1507–1510, 1973c.

    PubMed  Google Scholar 

  • Miller, J. F. A. P.: Studies on mouse leukaemia. The role of the thymus in leukaemogenesis. by cellfree leukaemic filtrates.Brit. J. Cancer 14: 93–98, 1960.

    PubMed  Google Scholar 

  • Oppltová, L. and Déniant, P.: Genetic determinants for graft versus host reaction in the H-2 complex.Transplant. Proc. 5: 1367–1372, 1973.

    PubMed  Google Scholar 

  • Rodt, H., Thierfelder, S., and Eulitz, M.: Anti-lymphocytic antibodies and marrow transplantation III. Effect of heterologous anti-brain antibodies on acute secondary disease in mice.Eur. J. Immunol. 4: 25–29, 1974.

    Google Scholar 

  • Sachs, D. H. and Cone, J. L.: A mouse B cell alloantigen determined by gene(s) linked to the major histocompatibility complex.J. Exp. Med. 138: 1289–1304, 1973.

    PubMed  Google Scholar 

  • Shreffler, D. C.: The Ss system of the mouse — a quantitative serum protein difference genetically controlled by the H-2 region.In J. Palm (ed.):Isoantigens and Cell Interaction, The Wistar Institute Press, Philadelphia, Pa., 1969, p. 11.

    Google Scholar 

  • Shreffler, D. C., David, D. S., Götze, D., Klein, J., McDevitt, H. O., and Sachs, D.: Genetic nomenclature for new lymphocyte antigens controlled by the Ir region of theH-2 complex.Immunogenetics, 1: 189–190, 1974.

    Google Scholar 

  • Stimpfling, J. H., and Reichert, A. E.: Strain C57BL/10ScSn and its congenic resistant sublines.Transplant. Proc. 2: 39–47, 1970.

    PubMed  Google Scholar 

  • Thorsby, E. and Bratlie, A.: A rapid method for the preparation of pure lymphocyte suspensions.In P. I. Terasaki (ed.):Histocompatibility Testing, 1970, Munksgaard, Copenhagen, 1970, p. 655.

    Google Scholar 

  • Vitetta, E. S., Baur, S., and Uhr, J. W.: Cell surface immunoglobulin II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes.J. Exp. Med. 134: 242–264, 1971.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Immunologic des Instituts für Hämatologie der Gesellschaft für Strahlen- und Umweltforschung, München, Germany

    Dietrich Götze

Authors
  1. Dietrich Götze
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Götze, D. T(Iat)- and B(Iab)-cell alloantigens determined by theH-2 linkedI region in mice. Immunogenetics 1, 495–506 (1974). https://doi.org/10.1007/BF01564087

Download citation

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01564087

Keywords

Search

Navigation