Abstract
C57BL/6 (H-2 b) mice and two mutants derived from this strain, B6.C-H-2 ba (Hz1) andE6-H-2 bd (M505), were studied in a number of functional tests, in vitro and in vivo, that assay for differences at theH-2 complex. All three strains give rise to reciprocal mixed lymphocyte reactivity (MLR) and cell-mediated lympholysis (CML) in vitro as well as graft-host reactivity (GVHR) and skin graft rejection in vivo. Analysis for cross-reactivity between these strains in CML revealed that the gained antigens in each mutant do not cross-react, and that Hz1 has lost an antigen shared by C57BL/6 and M505 strains. In addition, spleen cells from B10.A(4R) mice, which differ from theH-2 b haplotype only at theK end of theH-2 complex, recognize a common antigen shared by all three strains tested. Provided that the mutations occurred in theH-2K b gene, these data indicate that a) there are at least three antigenic specificities coded for by theH-2K b gene(s) that serve as targets for receptors on thymus-derived (T) cells in CML; b) since C57BL/6 strain mice and the mutants are serologically indistinguishable on a qualitative basis, the antigens recognized by the receptors on T cells and by humoral H-2 antibody are nonidentical; and c) mutation in theH-2K b locus itself can give rise to allogeneic recognition phenomena such as MLR and GVHR.
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Forman, J., Klein, J. Analysis ofH-2 mutants: Evidence for multiple CML target specificities controlled by theH-2K b gene. Immunogenetics 1, 469–481 (1974). https://doi.org/10.1007/BF01564085
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DOI: https://doi.org/10.1007/BF01564085