Abstract
The immune responses of nine inbred rat strains to a high dose of the synthetic copolymers GT, GA or GAT and of eight strains to low doses of BSA were compared. The strains tested represent six known haplotypes of the major histocompatibility locus in rats. The studies allowed a clear differentiation of the rat strains into high and low or nonresponders for GT and GA according to the levels of specific antibodies produced after primary and secondary immunization with these antigens and specific delayed type hypersensitivity reactions. A considerably less clear responder — non (or low) responder distribution pattern among the rat strains was found for GAT and low doses of BSA. The genetic background for the observed phenotypes and a possible relationship between responder status and the major histocompatibility locus are discussed.
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Abbreviations
- ABC:
-
antigen-binding capacity calculated as micrograms of antigen-bound per milliliter of undiluted serum
- BSA:
-
bovine serum albumin, fraction
- VDHR:
-
delayed hypersensitivity reaction
- GA:
-
random synthetic copolymer of L-glutamic acid50, L-alanine50, M.W. 45,000
- GAT:
-
random synthetic copolymer of L-glutamic acid60, L-alanine30, L-tyrosine10, M.W. 55,000
- GT:
-
random synthetic copolymer of L-glutamic acid50, L-tyrosine50, M.W. 22,000
- Ir gene:
-
immune response gene
- PBS:
-
phosphate-buffered saline, pH 7.3
- LDH:
-
lactic dehydrogenase
- (T,G)-A-L:
-
branched chain synthetic polymer of poly-L (tyrosine, glutamic acid)-poly-D,L-alanine-poly-L-lysine
- ACI:
-
ACI/fMaI
- AUG:
-
August 28807/Cr
- BN:
-
Brown Norway/fMaI
- BUF:
-
Buffalo/fMaI
- COP:
-
Copenhagen 233/Cr
- F344:
-
Fisher 344/fMaI
- LEW:
-
Lewis/fMaI
- M520:
-
Marshall 520/Cr
- WF:
-
Wistar Furth
References
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Armerding, D., Katz, D.H. & Benacerraf, B. Immune response genes in inbred rats. I. Analysis of responder status to synthetic polypeptides and low doses of bovine serum albumin. Immunogenetics 1, 329–339 (1974). https://doi.org/10.1007/BF01564071
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DOI: https://doi.org/10.1007/BF01564071