Abstract
The Ly-4.2 alloantigenic specificity has been described as a possible marker for B cells in the mouse, as, on testing by the lymphocytotoxicity method, there was a restricted distribution of this specificity on lymphocytes. Using the highly sensitive method of immunoelectron microscopy with a hybrid antibody, it was found that only 5.5% of cells in the thymus carried the Ly-4.2 specificity, compared with 55.7% in the spleen and 20.9% in lymph nodes. This distribution suggests that Ly-4.2 is present on B cells, and confirms other functional and population studies. However, by immunoelectron microscopy Ly-4.2 was also detected on a few macrophages in the thymus, spleen, and lymph nodes. As with other alloantigens, the location of Ly-4.2 was found in restricted areas of various sizes on the cells bearing this specificity.
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Aoki, T., McKenzie, I.F.C., Sturm, M.M. et al. Distribution of the alloantigen Ly-4.2 on murine B cells. Immunogenetics 1, 291–296 (1974). https://doi.org/10.1007/BF01564068
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DOI: https://doi.org/10.1007/BF01564068