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H-2-linked recessiveIr gene regulation of high antibody responsiveness to TNP hapten conjugated to autogenous albumin

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Abstract

The antibody response to the hapten 2,4,6-trinitrophenyl (TNP) conjugated to autogenous mouse serum albumin (MSA) is regulated by anIr gene(s) located within the major histocompatibility complex (MHC). Both the qualitative and quantitative ability of congenic strains to produce TNP-specific antibodies are functions of theH-2 haplotype. Thus, mouse strains may be classified as high (H-2 d), intermediate (H-2 b,H-2 s), and low responders (H-2 a,H-2 k,H-2 n,H-2 p,H-2 q). Antibody responses, as measured by antigen-binding capacities in modified Farr assays, were compared among strains carrying recombinantH-2 haplotypes and their hybrid progenies. Distinct high- and low-responder phenotypes were evident throughout the time course of both primary and secondary antibody responses. The gene locus controlling specific responsiveness to TNP-MSA, now designatedIr-6, was mapped within theI-B subregion of theH-2 complex. Recessive inheritance of high responsiveness was confirmed in hybrid progenies of three different low × high-responder crosses.

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Authors and Affiliations

  1. Immunogenetics Group, Department of Microbiology and Immunology, and Dental Research Institute, School of Medicine, University of California at Los Angeles, 90024, Los Angeles, California

    Walter J. Urba & William H. Hildemann

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  1. Walter J. Urba
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  2. William H. Hildemann
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Urba, W.J., Hildemann, W.H. H-2-linked recessiveIr gene regulation of high antibody responsiveness to TNP hapten conjugated to autogenous albumin. Immunogenetics 6, 433–445 (1978). https://doi.org/10.1007/BF01563935

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