Abstract
Peripheral blood leukocytes from normal volunteers and from patients with untreated Hodgkin's diseases have been assayed for the magnitude of their responses to pools of selected stimulating cells in a quantitative one-way mixed leukocyte reaction (MLR). Responses by leukocytes from individuals withHLA-B8 segregated above the median response regardless of whether the donors were healthy or were patients with Hodgkin's disease. These results suggest the presence of a gene (or genes) in the responding cells that has an effect on the magnitude of the MLR. This gene is in nonrandom association with the gene coding forB8 at theB region of theHLA complex. The observed linkage disequilibrium ofB8 with a gene controlling the proliferative response to alloantigens may be the basis for the increased frequency ofB8 in long-term survivors in carcinoma of the breast and Hodgkin's disease, and for the association ofB8 with several autoaggressive diseases.
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Abbreviations
- MLR:
-
mixed leukocyte reaction
- SPI, SPII, and SPIII:
-
selected pools of stimulating cells
- cpm:
-
counts per minute
References
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Osoba, D., Falk, J. HLA-B8 phenotype associated with an increased mixed leukocyte reaction. Immunogenetics 6, 425–432 (1978). https://doi.org/10.1007/BF01563934
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DOI: https://doi.org/10.1007/BF01563934