Abstract
An antigen reactive with antisera to a human chromosome 7-coded cell-surface antigen can be extracted from human cells with the nonionic detergent NP-40. Immune complexes of radiolabeled cell extracts analyzed on SDS polyacrylamide gels showed a single protein with an apparent M.W. of approximately 165,000. This protein was immunoprecipitated from extracts of hybrid cells containing chromosome 7 and human cell extracts, but not from mouse cell extracts or from extracts of a hybrid clone without human chromosome 7. The data presented here indicate that the protein identified on the gels is a cell-surface glycoprotein coded for by human chromosome 7.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aden, D.P. and Knowles, B.B.: Cell-surface antigens coded for by the human chromosome 7.Immunogenetics 3:209–211, 1976
Bonner, W.M. and Laskey, R.A.: A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels.Eur. J. Biochem, 46:83–88, 1974
Buck, D.W. and Bodmer, W.F.: The human species antigen on chromosome 11.Cytogenet. Cell Genet. 14:257–259, 1975
Buck, D.W., Goss, S.J., and Bodmer, W.F.: Regional mapping of the X-linked gene for a human cell surface antigen, SA-X.Cytogenet. Cell Genet. 16:99–100, 1976
Croce, C.M., Girardi, A.J., and Koprowski, H.: Assignment of the T-antigen gene of simian virus 40 to human chromosome C-7.Proc. Natl. Acad. Sci. U.S.A. 70:3617–3620, 1973
Croce, C.M. and Koprowski, H.: Somatic cell hybrids between mouse peritoneal macrophages and SV40-transformed human cells. I. Positive control of the transformed phenotype by the human chromosome 7 carrying the SV40 genome.J. Exp. Med. 140:1221–1229, 1974
Cullen, S.E. and Schwartz, B.D.: An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci.J. Immunol. 117:136–142, 1976
Davies, G.E. and Stark, G.R.: Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.Proc. Natl. Acad. Sci. U.S.A. 66:651–656, 1970
Goodfellow, P.N., Jones, E.A., Van Heynigen, V., Solomon, E., Bobrow, M., Miggiano, V., and Bodmer, W.F.: The β2-microglobulin gene is on chromosome 15 and not in the Hl-A region.Nature 54:261–269, 1975
Hynes, R.O.: Alteration of cell-surface proteins by viral transformation and by proteolysis.Proc. Natl. Acad. Sci. U.S.A. 70:3170–3174, 1973
Jha, K.K. and Ozer, H.L.: Expression of transformation in cell hybrids. I. Isolation and application of density-inhibited BALB/3T3 cells deficient in hypoxanthine phosphoribosyltransferase and resistant to ouabain.Som. Cell Genet. 2:215–223, 1976
Khoury, G. and Croce, C.M.: Quantitation of the viral DNA present in somatic cell hybrids between mouse and SV40-transformed human cells.Cell 6:535–542, 1975
Knowles, B.B., Solter, D., Trinchieri, G., Maloney, K.M., Ford, S.R., and Aden, D.P.: Complementmediated antiserum cytotoxic reactions to human chromosome 7 coded antigen(s): immunoselection of rearranged human chromosome 7 in human-mouse somatic cell hybrids.J. Exp. Med. 145:314–326, 1977a
Knowles, B.B., Ford, S.R., and Aden, D.P.: Reactivity to SV40 T antigen in athymic (nude), antithymocyte serum-treated, and normal mice.J. Immunol. 119:79–82, 1977b
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227:680–685, 1970
McAllister, R.M., Melnyk, J., Finkelstein, J.Z., Adams, Jr., E.C., and Gardner, M.B.: Cultivation in vitro of cells derived from a human rhabdomyosarcoma.Cancer 24:520–526, 1969
McKusick, V.A.: Human gene mapping 3.Baltimore Conference (1975) Third International Workshop on Human Gene Mapping, pp. 1–6. Birth defects and original article series. The National Foundation-March of Dimes XII, #7, Karger, New York, 1976
Moore, E.E., Jones, C., and Puck, T.T.: Cell-surface antigens. IV. Immunological correspondence betwen glycophorin and the a1 human cell-surface antigen.Cytogenet. Cell Genet. 17:89–97, 1976
Puck, T.T., Wuthier, P., Jones, C., and Kao, F.T.: Genetics of somatic mammalian cells: lethal antigens as genetic markers for study of human linkage groups.Proc. Natl. Acad. Sci. U.S.A. 68:3102–3106, 1971
Rasheed, S., Nelson-Rees, W.A., Toth, E.M., Arnstein, P., and Gardner, M.B.: Characterization of a newly derived human sarcoma cell line (HT-1080).Cancer 33:1027–1033, 1974
Revel, M., Bash, D., and Ruddle, F.H.: Antibodies to a cell-surface component coded by human chromosome 21 inhibit action of interferon.Nature 260:139–141, 1976
Strand, M., August, J.T., and Croce, C.M.: Selective induction of murine oncornavirus gene expression in somatic cell hybrids between mouse peritoneal macrophages and SV-40 transformed human cells.Virology 70:545–549, 1976
Trinchieri, G., Aden, D.P., and Knowles, B.B.: Cell-mediated cytotoxicity to SV40-specific tumor-associated antigens.Nature 261:312–314, 1976
Van Someren, H., Westerveld, A., Hagemeíjer, A., Mees, J.R., Meera Kahn, P., and Zaalberg, O.B.: Human antigen and enzyme markers in man-Chinese hamster somatic cell hybrids: evidence for synteny between the HL-A, PGM3, ME1, and IPO-B loci.Proc. Natl. Acad. Sci. U.SA. 71:962–965, 1974
Weiss, M.C., Ephrussi, B., and Scaletta, L.J.: Loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells.Proc. Natl. Acad. Sci. U.S.A. 59:1132–1135, 1968
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ford, S.R., Aden, D.P., Mausner, R. et al. Partial characterization of cell-surface protein coded for by human chromosome 7. Immunogenetics 6, 293–300 (1978). https://doi.org/10.1007/BF01563921
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01563921