Abstract
BALB/cBy anti-BALB/cJ spleen cells were tested in a secondary cellmediated lympholysis assay. The effector cells generated displayed a positive cytotoxic effect against Con A lymphoblasts from only those strains that were typed serologically as having theQa-2 a allele. Confirmation that the target antigen is controlled by a locus closely associated with or identical toQa-2 was obtained by the findings that target cells from B6.K2 (Qa-2 a,Qa-3 a) mice were lysed by the effector cells, while those from theQa-2, 3 congenic strain B6.K1 (Qa-2 b,Qa-3 b) were not. The fact that target cells from aQa-2-positive/Qa-3-negative strain (DBA/1,Qa-2 ai,Qa-3 b) were killed indicates that the target antigen is controlled, at least in part, by theQa-2 locus, not the Qa-3.There is no observedH-2 genetic restriction for this cytotoxic effect, since target cells which have theQa-2 a allele but differ from the stimulator cells at theH-2K, D, andI regions were lysed efficiently.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alter, B.J., Schendel, D.J., Bach, M.L., Bach, F.H., Klein, J., and Stimpfling, J.H.: Cell-mediated lympholysis. Importance of serologically denned H-2 regions.J. Exp. Med. 137:1303–1309, 1973
Bevan, M.J.: Alloimmune cytotoxic T cells: evidence that they recognize serologically denned antigens and bear clonally restricted receptors.J. Immunol. 114:316–319, 1975
de Landazuri, M.O. and Herberman, R.B.: Specificity of cellular immune reactivity to virus-induced tumors.Nature (New Biol.) 238:18–19, 1972
Flaherty, L.: TheTla region of the mouse: identification of a new serologically defined locus,Qa-2. Immunogenetics 3:533–539, 1976
Flaherty, L., Sullivan, K., and Zimmerman, D.: TheTla locus: a new allele and antigenic specificity.J. Immunol. 119:571–575, 1977
Flaherty, L., Zimmerman, D., and Hansen, T.H.: Further serological analysis of the Qa antigens. Analysis of an anti-H-2-28 serum.Immunogenetics 6:245–251, 1978
Forman, J.; On the role of theH-2 histocompatibility complex in determining the specificity of cytotoxic effector cells sensitized against syngeneic trinitrophenyl-modified target cells.J. Exp. Med. 142:403–418. 1975
Forman, J. and Klein, J.: Analysis of H-2 mutants: evidence for multiple CML target specificities controlled by the H-2Kb gene.Immunogenetics 1:469–481, 1975
Gordon, R.D., Simpson, E., and Samelson, L.E.:In vitro cell-mediated immune responses to the male specific (H-2) antigen in mice.J. Exp. Med. 142:1108–1120, 1975
Hayry, P.: Review: anamnestic responses in mixed lymphocyte culture-induced cytolysis (MLC-CML) reactions.Immunogenetics 3:417–453, 1976
Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. Springer-Verlag, New York, 1975
Klein, J. and Chiang, C.: A CML locus (Qa-1) at theD end of theH-2 complex.Immunogenetics 6:235–243, 1978
Klein, J., Geib, R., Chiang, C., and Hauptfeld, V.: Histocompatibility antigens controlled by theI region of the murineH-2 complex. I. Mapping ofH-2A andH-2C loci.J. Exp. Med. 143:1439–1452, 1976
Melvold, R.W. and Kohn, H.I.: Eight new histocompatibility mutations associated with theH-2 complex.Immunogenetics 3:185–191, 1976
Nabholz, M., Vives, J., Young, H.M., Meo, T., Miggiano, V., Rijnbeek, A., and Shreffler, D.C.: Cell mediated lysisin vitro: genetic control of killer cell production and target specificities in the mouse.Eur. J. Immunol. 4:378–387, 1975a
Nabholz, M., Young, H., Rijnbeek, A., Boccardo, R., David, C.S., Meo, T., Miggiano, V., and Shreffler, D.C.: I-region associated determinants: expression on mitogen-stimulated lymphocytes and detection by cytotoxic T cells.Eur. J Immunol. 5:594–599, 1975b
Shearer, G.M., Rehn, R., and Garbarino, C.A.: Cell-mediated lympholysis of trinitrophenyl-modified autologous lymphocytes. Effector cell specificity to modified cell surface components controlled by theH-2K andH-2D serological regions of the murine major histocompatibility complex.J. Exp. Med. 141:1348–1364, 1975
Stanton, T.H. and Boyse, E.A.: A new serologically defined locus,Qa-1, in theTla region of the mouse.Immunogenetics 3:525–531, 1976
Wagner, H., Gotze, D., Ptschelinzew, L., and Röllinghoff, H.: Induction of cytotoxic T lymphocytes againstI region coded determinants:in vitro evidence for a third histocompatibility locus in the mouse.J. Exp. Med. 142: 1477–1487, 1975
Zinkernagel, R.M. and Doherty, P.C.: H-2 compatibility requirement for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. Different cytotoxic T-cell specificities are associated with structures coded for H-2K or H-2D.J. Exp. Med. 141:1427–1436, 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Forman, J., Flaherty, L. Identification of a new CML target antigen controlled by a gene associated with theQa-2 locus. Immunogenetics 6, 227–233 (1978). https://doi.org/10.1007/BF01563912
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01563912