Skip to main content
SpringerLink
Log in

Genetically controlled IgM hyporesponsiveness to aK. pneumoniae polysaccharide

  • Original Investigations
  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

Examination of the immune response to aKlebsiella pneumoniae polysaccharide (K47-PS) has revealed that BALB/c mice demonstrate only a very weak primary response to this antigen. The low response does not result from either a peculiar dose response curve for BALB/c mice or from differing optimal antigen concentrations for high and low responder mice. Genetic analysis indicates that this variability of response is explicable assuming two alleles at a single locus; high responsiveness is dominant. Variability of response is probably not linked to theH-2 complex since the low and high responder mice, BALB/c and B10.D2/Sn new line, respectively, share the sameH-2 haplotype (H- 2d). Tests of F2s, backcrosses, and appropriate congenics have not shown evidence of linkage to sex, the albinism gene, the genes controlling coat color (agouti, black, brown), or the allotype-constant-region genes. The hyporesponsiveness is apparent only in the primary (IgM) response; hyperimmunization evokes similar antibody titers in high and low responding strains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Amsbaugh, D.F., Hansen, C.T., Prescott, B., Stashak, P.W., Barthold, D.R., and Baker, P.J.: Genetic control of the antibody response to Type III pneumococcal polysaccharide in mice. I. Evidence that an x-linked gene plays a decisive role in determining responsiveness.J. Exp. Med. 136:931–949, 1972

    PubMed  Google Scholar 

  • Benacerraf, B., Ojeda, A., and Maurer, P.H.: Studies on artificial antigens. II. The antigenicity in guinea pigs of arsanilic acid conjugates of copolymers of d- or L-a-amino acids.J. Exp. Med. 118:945:952, 1963

    PubMed  Google Scholar 

  • Berzofsky, J.A., Schechter, A.N., Shearer, G.M., and Sachs, D.H.: Genetic control of the immune response to staphylococcal nuclease. III. Time-course and correlation between the response to native nuclease and the response to its polypeptide fragments.J. Exp. Med. 145:111–122, 1977

    PubMed  Google Scholar 

  • Braley-Mullen, H. and Sharp, G.C.: Conversion of Type III pneumococcal polysaccharide low responders to high responders by immunization with a thymus-dependent form of antigen.Cell. Immunol. 12:49–60, 1974

    PubMed  Google Scholar 

  • Braun, D.G., Kindred, B., and Jacobson, E.B.: Streptococcal group A carbohydrate antibodies in mice: evidence for strain differences in magnitude and restriction of the response and for thymus dependence.Eur. J. Immunol. 2:138–143, 1972

    PubMed  Google Scholar 

  • Cunningham, A.J. and Szenberg, A.: Further improvements in the plaque technique for determining single-antibody forming cells.Immunol. 14:599–600, 1968

    Google Scholar 

  • Goding, J.W.: The chromic chloride method of coupling antigens to erythrocytes: definition of some important parameters.J. Immunol. Methods 10:61–66,1976

    PubMed  Google Scholar 

  • Grumet, F.C.: Genetic control of the immune response. A selective defect in immunologic (IgG) memory in nonresponder mice.J. Exp. Med. 135:110–125, 1972

    PubMed  Google Scholar 

  • Krause, R.M.: The search for antibodies with molecular uniformity.Adv. Immunol. 12:1–56, 1970.

    PubMed  Google Scholar 

  • Lovchik, J.C. and Diener, E.: Genetic control of antibody response to a thymus independent antigen linked to the major histocompatibility complex.Fed. Proc. 36:1195, 1977

    Google Scholar 

  • McDevitt, H.O.: Genetic control of the antibody response. III. Quantitative and qualitative characterization of the antibody response to (T,G)-A-L in CBA and C57 mice.J. Immunol. 100:485–492, 1968

    PubMed  Google Scholar 

  • McDevitt, H.O. and Benacerraf, B.: Genetic control of specific immune responses.Adv. Immunol. 11:31–74, 1969

    PubMed  Google Scholar 

  • Rosenberg, L.T. and Tachibana, D.K.: On mouse complement: genetic variants,J. Immunol. 103:1143–1148, 1969

    PubMed  Google Scholar 

  • Rosenthal, A.S., Barcinski, M.A., and Blake, J.T.: Determinant selection is a macrophage dependent immune response gene function.Nature 267:156–158, 1977

    Google Scholar 

  • Sandford, P.A. and Conrad, H.E.: The structure of theAerobacter aerogenes A3(S1)polysaccharide. I. A reexamination using improved procedures for methylation analysis.Biochemistry 5:1508–1517, 1966

    PubMed  Google Scholar 

  • Tachibana, D.K. and Rosenberg, L.T.: Characterization of a genetically identifiable component of complement in mice.Folia Microbiol. (Praha)9:129–143, 1964

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Stanford University School of Medicine, 94305, Stanford, California

    Mary E. White-Scharf & Leon T. Rosenberg

Authors
  1. Mary E. White-Scharf
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leon T. Rosenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

White-Scharf, M.E., Rosenberg, L.T. Genetically controlled IgM hyporesponsiveness to aK. pneumoniae polysaccharide. Immunogenetics 6, 81–89 (1978). https://doi.org/10.1007/BF01563898

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01563898

Keywords

Search

Navigation