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Soluble and membrane-associated heat shock proteins in soybean root

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Summary

Localization of heat shock proteins (Hsp) in endomembranes and determination of whether they are integral or peripheral membrane proteins will aid in understanding the physiological function of the heat shock response. Radiolabeled endomembranes (endoplasmic reticulum, Golgi, and plasma membrane), obtained by sucrose gradient centrifugation of heat-shocked soybean (Glycine max L.) root tissue were solubilized and the polypeptides separated by two-dimensional IEF-SDS-PAGE. Autoradiography revealed three groups of Hsp. A diverse group fo 25 low mol wt Hsp (18 to 24 kDa) with isoelectric point (pI) between 5 and 7; an intermediate mol wt group (30 to 47 kDa) with pI of 5.5 to 6.0; and a group of two high mol wt Hsp (75 to 80 kDa) with pI 4.8 to 5.2. The plasma membrane fraction lacked the Hsp pair of 47 kDa detected in the endoplasmic reticulum and Golgi fractions but possessed a unique Hsp of 30 kDa, pI 5.5.

Comparison of soluble and microsome fractions revealed a difference in the pattern of the low mol wt Hsp class. The soluble fraction contained Hsp of 16–20 kDa with pI between 5 and 7.8 while the microsome fraction was characterized by Hsp of 18–24 kDa with pI between 5.8 and 6.5.

The microsomal Hsp were not released by 1 M KCl. Treatment of the microsome fraction with Triton X-100 selectively released several Hsp, and Na2CO3 treatment removed additional Hsp from the membrane fraction.

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Abbreviations

Hsp:

heat-shock protein(s)

GA:

Golgi apparatus

PM:

plasma membrane

2 D:

two-dimensional

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Author notes

  1. P. R. LaFayette

    Present address: Department of Botany, University of Georgia, Athens, Georgia, USA

Authors and Affiliations

  1. Department of Agronomy and Range Science, University of California, Davis, California

    P. R. LaFayette & R. L. Travis

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  1. P. R. LaFayette
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  2. R. L. Travis
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LaFayette, P.R., Travis, R.L. Soluble and membrane-associated heat shock proteins in soybean root. Protoplasma 156, 174–182 (1990). https://doi.org/10.1007/BF01560655

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